| CXCR4-siRNA逆转录病毒载体对宫颈癌Caski细胞CXCR4基因表达的抑制作用 |
| |
| 引用本文： | 康楷, 胡丽娜, 董晓静, 朱元方. CXCR4-siRNA逆转录病毒载体对宫颈癌Caski细胞CXCR4基因表达的抑制作用[J]. 中国肿瘤临床, 2008, 35(21): 1244-1248. |
| |
| 作者姓名： | 康楷 胡丽娜 董晓静 朱元方 |
| |
| 作者单位： | 1.重庆医科大学附属第一医院妇产科, 重庆市 610041;;2.四川大学华西医学中心第二医院妇产科;;3.重庆医科大学附属第二医院妇产科 |
| |
| 摘 要： | 目的: 构建针对趋化因子受体CXCR4的RNA干扰逆转录病毒载体,检测由逆转录病毒介导的RNA干扰对人宫颈癌Caski细胞CXCR4基因表达的抑制作用. 方法: 人工合成CXCR4特异性小干扰RNA(small interfering RNA,siRNA)片段,将其插入带有绿色荧光蛋白(EGFP)的逆转录病毒载体质粒pSOS,测序正确后进行重组,转染PT67细胞包装成病毒,收获病毒上清用其转染Caski细胞,采用实时定量PCR和Western blot分别从mRNA水平和蛋白水平检测其对Caski细胞CXCR4表达的抑制作用. 结果: 成功构建pSOS-siCXCR4逆转录病毒载体,筛选出高效抑制序列,与对照组比较,在Caski细胞的mRNA水平,CXCR4-siRNA在24h、48h和72h对CXCR4 mRNA表达的抑制率分别为29.9%、56.8%和62.8%,而在蛋白水平对CXCR4蛋白的抑制率分别为43.6%、49.6%和62.9%. 结论: pSOS-HUS-siCXCR4干扰载体可以有效抑制Caski细胞中CXCR4表达.
|
| 关 键 词： | RNA干扰 CXCR4 逆转录病毒 宫颈癌 Caski细胞 |
| 收稿时间： | 2008-06-10 |
Down-regulation of CXCR4 by Inducible Retrovirally Mediated RNA Interference Inhibits Human Cervix Carcinoma Cell Invasion in Vitro |
| |
| KANG Kai, HU Li-na, DONG Xiao-jing, ZHU Yuan-fang. Down-regulation of CXCR4 by Inducible Retrovirally Mediated RNA Interference Inhibits Human Cervix Carcinoma Cell Invasion in Vitro[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2008, 35(21): 1244-1248. |
| |
| Authors: | KANG Kai HU Lina DONG Xiaojing ZHU Yuanfang |
| |
| Affiliation: | 1.Department of O&G, The Frist Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China;;2.Department of O&G, The Second Affiliated Hospital of Huaxi Medical Centre, Sichuan University, 610041, China;;3.Department of O&G, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China |
| |
| Abstract: | Objective: It is suggested that the chemokine receptor CXCR4 plays an important role in the invasion and metastasis of cervical cancer, but there are few published articles about down-regulating the CXCR4 expression to prevent the metastasis of uterine cervix cancer. The aim of this study was to construct RNA inter- fering retrovirus vector of CXCR4 and to investigate the expression of CXCR4 receptor in human cervix carci- noma Caski cells interfered with retrovirally mediated RNA interference targeting CXC... |
| |
| Keywords: | CXCR4 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国肿瘤临床》浏览原始摘要信息 |
|
点击此处可从《中国肿瘤临床》下载全文 |
|
