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| 追毒方逆转三阴性乳腺癌MDA-MB-231细胞耐药的作用机制 | |
| 引用本文： | 刘敏,刘春亮,梁国强,姚霏,张晓迪,陈婷,袁雅琴,吴士良. 追毒方逆转三阴性乳腺癌MDA-MB-231细胞耐药的作用机制[J]. 南京中医药大学学报, 2019, 35(1): 63-67 |
| 作者姓名： | 刘敏 刘春亮 梁国强 姚霏 张晓迪 陈婷 袁雅琴 吴士良 |
| 作者单位： | 1.南京中医药大学附属苏州市中医医院，江苏 苏州 215009 |
| 摘 要： | 目的 基于糖基转移酶β3GnT8探讨追毒方逆转三阴性乳腺癌MDA-MB-231细胞耐药的作用机制。方法 通过MTT法观察追毒方对MDA-MB-231耐药细胞增殖的抑制作用，通过转染β3GnT8得到高表达β3GnT8的MDA-MB-231/TAX细胞株，采用Western blot观察糖基转移酶β3GnT8和耐药蛋白BCRP、P-gp的表达，通过凝集素流式细胞术观察转染β3GnT8 MDA-MB-231/TAX细胞膜表面β3GnT8的荧光强度。结果 追毒方有效成分能明显抑制三阴性乳腺癌MDA-MB-231耐药细胞的增殖，作用72 h抑制率达到50.16%，能有效下调β3GnT8和耐药蛋白BCRP、P-gp的表达，并且β3GnT8的表达与BCRP、P-gp呈正相关。结论 追毒方可通过下调糖基转移酶β3GnT8，从而降低耐药蛋白BCRP、P-gp的表达，逆转三阴性乳腺癌MDA-MB-231细胞耐药性。 |
| 关 键 词： | 追毒方 三阴性乳腺癌MDA-MB-231耐药细胞 β3GnT8 逆转耐药 |
Reverse Effect and Mechanism of Effective Components of Zhuidu Fang on Triple Negative Breast Cancer MDA-MB-231 Drug-Resistant Cells |
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| LIU Min,LIU Chun-liang,LIANG Guo-qiang,YAO Fei,ZHANG Xiao-die,CHEN Ting,YUAN Ya-qin,WU Shi-liang. Reverse Effect and Mechanism of Effective Components of Zhuidu Fang on Triple Negative Breast Cancer MDA-MB-231 Drug-Resistant Cells[J]. Journal of Nanjing University of Traditional Chinese Medicine(Natural Science), 2019, 35(1): 63-67 | |
| Authors: | LIU Min LIU Chun-liang LIANG Guo-qiang YAO Fei ZHANG Xiao-die CHEN Ting YUAN Ya-qin WU Shi-liang |
| Affiliation: | 1.Affiliated Suzhou Hospital of TCM to Nanjing University of Chinese Medicine, Suzhou, 215009, China2.School of Biology and Basic Medical Sciences, Soochow University, Suzhou, 215123, China |
| Abstract: | OBJECTIVE Based on glycosyltransferase β3GnT8， to investigate the mechanism of reverse effect on triple negative breast cancer MDA-MB-231 drug-resistance cells by Zhuidu Fang. METHODS The inhibition of MDA-MB-31 drug-resistance cells proliferation by Zhuidu Fang was detected by MTT assay. By interference and transfection of β3GnT8， high and low expressed β3GnT8 MDA-MB-231/TAX cell lines were obtained. Western blot was used to detect the protein expression of β3GnT8， and drug resistance protein BCRP and P-gp. Lectin flow cytometry was used to detect β3GnT8 fluorescence intensity on β3GnT8 transfected MDA-MB-231/TAX cells. RESULTS Zhuidu Fang effectively inhibited the proliferation of MDA-MB-231/TAX cells， and the inhibitory rate was 50.16% after 72 h. Zhuidu Fang significantly downregulated the protein expression of β3GnT8， BCRP and P-gp. The protein expression of β3GnT8 showed a positive correlation with BCRP and P-gp. CONCLUSION Zhuidu Fang can reverse the drug-resistance of MDA-MB-231 cell lines， which is associated with the downregulation of the expression of β3GnT8， BCRP and P-gp. |
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