PCR-based diagnosis of Naegleria sp. infection in formalin-fixed and paraffin-embedded brain sections |
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| Authors: | Schild Marc Gianinazzi Christian Gottstein Bruno Müller Norbert |
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| Affiliation: | Institute of Parasitology, University of Berne, L?nggass-Strasse 122, CH-3012 Bern, Switzerland. |
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| Abstract: | We developed a real-time PCR which allowed the highly sensitive detection of Naegleria fowleri in histological brain tissue sections from experimentally infected mice. This genus-specific small-subunit (18S) rRNA gene-based PCR can complement conventional (immuno-) histology for the diagnosis of primary amoebic meningoencephalitis in paraffin-embedded brain necropsy specimens that had been fixed in formalin buffered with phosphate-buffered saline. |
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