Interleukin-12 is involved in the enhancement of human natural killer cell activity by Lactobacillus casei Shirota |
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| Authors: | Takeda K Suzuki T Shimada S-I Shida K Nanno M Okumura K |
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| Affiliation: | Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan. |
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| Abstract: | We conducted a placebo-controlled, cross-over trial to examine the effect of Lactobacillus casei Shirota (LcS) on natural killer (NK) cell activity in humans. NK cell activity exhibited a declining trend during the period of placebo ingestion, but NK cell activity increased after intake for 3 weeks of fermented milk containing 4 x 10(10) live LcS. When human peripheral blood mononuclear cells were cultured in the presence of heat-killed LcS, NK cell activity was enhanced. The ability of LcS to enhance NK cell activity and induce interleukin (IL)-12 production was correlated, and the addition of anti-IL-12 monoclonal antibody reduced the enhancement of NK cell activity triggered by LcS. In addition, separation of NK cells from LcS-stimulated monocytes with membrane filter reduced NK cell activity to the intermediate level and almost deprived monocytes of the ability to produce IL-12. These results demonstrate that LcS can enhance NK cell activity in vivo and in vitro in humans, and IL-12 may be responsible for enhancement of NK cell activity triggered by LcS. |
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| Keywords: | cell‐to‐cell contact IL‐12 Lactobacillus casei NK cell activity |
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