Amplifiable messenger RNA. |
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| Authors: | Y Wu D Y Zhang F R Kramer |
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| Affiliation: | Department of Molecular Genetics, Public Health Research Institute, New York, NY 10016. |
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| Abstract: | RNA molecules were prepared that consisted of an mRNA encoding chloramphenicol acetyltransferase embedded within the sequence of midivariant RNA, which is a template for the RNA-directed RNA polymerase Q beta replicase. These recombinant RNAs were shown to be bifunctional: they are amplified exponentially by incubation with Q beta replicase, and the replicated RNA serves as template for the cell-free synthesis of enzymatically active chloramphenicol acetyltransferase. The availability of amplifiable mRNAs will enable relatively large amounts of protein to be synthesized in vitro. |
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