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Twist ARF和E-cadherin在大肠癌组织中的表达及临床意义
引用本文:张娟, 魏君, 王士杰, 于跃明, 杨珊, 王贵英. Twist ARF和E-cadherin在大肠癌组织中的表达及临床意义[J]. 中国肿瘤临床, 2011, 38(3): 143-147 . DOI: 10.3969/j.issn.1000-8179.2011.03.006
作者姓名:张娟  魏君  王士杰  于跃明  杨珊  王贵英
作者单位:石家庄市河北医科大学第四医院外二科
摘    要:目的:探讨Twist、ARF、E-cadherin蛋白在大肠癌组织中的表达与临床病理参数的关系。同时分析Twist、ARF、 E-cad?herin与大肠癌生长,侵袭转移之间的关系。方法:采用免疫组织化学SP染色方法对60例大肠癌标本及60例正常大肠黏膜标本分别进行Twist、ARF、E-cadherin蛋白检测。结果:Twist在肿瘤组织中的阳性表达率明显高于正常大肠黏膜(P<0.05);ARF及E-cadherin在肿瘤组织中的阳性表达率明显低于正常大肠黏膜 (P<0.05),差异有统计学意义。Twist的表达与大肠癌的病理学分级、肠壁浸润深度、有无淋巴结转移、TNM分期相关 (P<0.05)。ARF、 E-cadherin的表达与大肠癌的病理学分级、肠壁浸润深度、有无淋巴结转移、 TNM分期、 有无远处转移相关 (P<0.05)。Twist与ARF共同阳性者10例,共同阴性者8例,两者呈显著性负相关 (r=0.806, P=0.004)。Twist与E-cadherin共同阳性者3例,共同阴性者7例,两者呈显著性负相关 (r=0.754, P=0.006)。结论:Twist的过度表达,ARF、E-cadherin蛋白的表达缺失在大肠癌的发生及侵袭转移中起重要的作用, Twist通过调节ARF/MDM2/p53途径抑制细胞凋亡促进肿瘤细胞形成, 同时影响E-cadherin的表达而促进上皮-间质转化现象,参与大肠癌的发生和转移。

关 键 词:大肠癌  Twist ARF E-cadherin  免疫组织化学
收稿时间:2010-09-16
修稿时间:2010-11-02

Expression of Twist, ARF and E-cadherin and Its Clinical Significance in Colorectal Cancer
Juan ZHANG,Jun WEI,Shijie WANG,Yueming YU,Shan YANG,Guiying WANG. Expression of Twist, ARF and E-cadherin and Its Clinical Significance in Colorectal Cancer[J]. Chinese Journal of Clinical Oncology, 2011, 38(3): 143-147. DOI: 10.3969/j.issn.1000-8179.2011.03.006
Authors:Juan ZHANG  Jun WEI  Shijie WANG  Yueming YU  Shan YANG  Guiying WANG
Abstract:Expression of Twist, ARF and E-cadherin and Its Clinical Significance in Colorectal CancerJuan ZHANG, JunWEI, ShijieWANG, Yueming YU, Shan YANG, GuiyingWANGCorrespondence to: GuiyingWANG, E-mail: tizq12@vip.163.comThe Second Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, ChinaAbstract Objective: To study the correlation of the expression of Twist, ARF and E-cadherin with the clinicopathologic parame-ters of colorectal cancer, and to analyze the relationships among Twist, ARF and E-cadherin proteins and tumorigenesis, invasion andmetastasis of colorectal cancer. Methods: Immunohistochemistry staining (SP) was conducted to detect the expression of Twist, ARFand E-cadherin protein in 60 samples of colorectal tumors and 60 samples of normal colorectal mucosa. Results: The positive rate ofTwist was significantly higher in the carcinoma samples than in the normal colorectal mucosa ( P < 0.05 ). The positive rate of ARF andE-cadherin was significantly lower in the carcinoma samples than in the normal colorectal mucosa ( P < 0.05 ). The expression of Twistwas correlated with the pathological grading of the colorectal cancer, the depth of tumor infiltration into the wall of the large intestine,the presence of lymph node metastases and TNM staging ( P < 0.05). The expression of ARF and E-cadherin was correlated with patho-logical grade of the cancer, depth of tumor infiltration, the presence of lymph node metastases, TNM stage and the presence of distantmetastasis ( P < 0.05 ). There were 10 cases with equally positive expression of Twist and ARF in the colorectal carcinoma group and 8with equally negative expression. There was a significant negative correlation between Twist and ARF expression in the carcinoma sam-ples ( r = 0.806, P = 0.004 < 0.05 ). There were 3 colorectal carcinoma cases with simultaneous positive expression of Twist and E-cad-herin and 7 with simultaneous negative expression. There was a remarkable negative correlation between Twist and E-cadherin expres-sion in colorectal carcinoma ( r = 0.754, P = 0.006 < 0.05). Conclusion: The overexpression of Twist and underexpression of ARF andE-cadherin may play an important role in the tumorigenesis, invasion and metastasis of colorectal cancer. Twist can inhibit apoptosisand promote tumor cell formation by regulating the ARF/MDM2/P53 pathway. Twist can also simultaneously affect the expression ofE-cadherin and promote epithelial mesenchymal transition (EMT), thus participating in the genesis and metastasis of colorectal cancer.Keywords Colorectal cancer; Twist; ARF; E-cadherin; Immunohistochemistry
Keywords:Twist  ARF  E-cadherin
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