| 人附睾蛋白4在子宫内膜癌组织及血清中的表达及临床意义 |
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| 引用本文： | 杨彩虹, 徐永辉, 张雪玉. 人附睾蛋白4在子宫内膜癌组织及血清中的表达及临床意义[J]. 中国肿瘤临床, 2011, 38(9): 516-519 . DOI: 10.3969/j.issn.1000-8179.2011.09.009 |
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| 作者姓名： | 杨彩虹 徐永辉 张雪玉 |
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| 作者单位： | 银川市宁夏医科大学附属医院妇科 |
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| 基金项目： | 本文课题受宁夏回族自治区科技攻关计划项目基金 |
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| 摘 要： | 目的：探讨人附睾蛋白4 （HE4） 在子宫内膜癌组织及血清中的表达及临床意义。方法：选取2009年9月至2010年3月宁夏医科大学附属31例子宫内膜癌患资料， 用免疫组织化学法检测正常对照组 （正常增生期子宫内膜） 20例， 子宫内膜增生组19例及子宫内膜癌组31例组织中HE4蛋白的表达水平， 检测结果用image pro-plus 6.0专业图像分析软件进行定量分析， 选择测定目标积分光密度 （IOD） 作为评价参数。应用酶联免疫吸附试验检测正常对照组 （正常增生期子宫内膜） 20例， 子宫内膜增生组19例及子宫内膜癌组31例患者血清中HE4的水平。结果： 1） HE4在子宫内膜癌组的表达高于正常对照组及子宫内膜增生组， 差异有统计学意义 （P<0.001）。HE4在子宫内膜癌Ⅰ期组织中的IOD中位数低于Ⅱ、 Ⅲ期组织中IOD中位数， 差异有统计学意义 （P<0.001）。HE4在子宫内膜癌组织中IOD与FIGO分期呈正相关关系 （P<0.001）。2） 子宫内膜癌组HE4的血清水平均高于正常对照组及子宫内膜增生组， 差异有统计学意义 （P<0.05）； 子宫内膜癌组术后血清中HE4的表达水平明显低于术前 （P<0.05）； 3） 子宫内膜癌组组织中的表达和术前血清中的水平呈正相关关系； 4） 子宫内膜癌组术前血清HE4水平与FIGO分期、 血清CA125呈正相关关系。结论： 子宫内膜癌组织及血清HE4表达升高， 可作为子宫内膜癌血清学标志物， 是子宫内膜癌的早期辅助诊断、 疗效评价、预后评估的客观指标， 可用于指导临床的综合治疗。
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| 关 键 词： | 子宫内膜癌 人附睾蛋白4 目标积分光密度 |
| 收稿时间： | 2010-12-10 |
| 修稿时间： | 2011-03-14 |
Expression and Significance of Human Epididymis Gene Product 4 in Tissue and Serum of Patients with Endometrial Cancer |
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| Caihong YANG,Yonghui XU,Xueyu ZHANG. Expression and Significance of Human Epididymis Gene Product 4 in Tissue and Serum of Patients with Endometrial Cancer[J]. Chinese Journal of Clinical Oncology, 2011, 38(9): 516-519. DOI: 10.3969/j.issn.1000-8179.2011.09.010 |
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| Authors: | Caihong YANG Yonghui XU Xueyu ZHANG |
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| Abstract: | Expression and Significance of Human Epididymis Gene Product 4 in Tissue and Serum ofPatients with Endometrial CancerCaihong YANG1, Yonghui XU2, Xueyu ZHANG1Correspondence to: Xueyu ZHANG, E-mail: zhxueyu@163.com1Department of Gynecology, The Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, China2Department of Pathology, The Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, ChinaThis study was supported by funds from 2010 Torch-Plan Projects of Ningxia Hui Autonomous Region (No. Ningkejizi[2010]168)Abstract Objective: To explore expression and significance of human epididymis protein 4 ( HE4 ) in tissue and serum of pa-tients with endometrial cancer. Methods: Immunohistochemistry was used to detect the expression of HE4 protein in the tissue of thenormal control ( NC ) group with normal proliferative endometrium ( n = 20 ), the endometrial hyperplasia ( EnHy ) group ( n = 19 )and endometrial carcinoma ( EnCa ) group ( n = 31 ). Quantitative analysis of the detection was conducted by the pro-plus 6.0 profes-sional image analysis software, and the target integral optical density ( IOD ) was used for the judgement parameter. The enzyme-linkedimmuno-sorbent assay ( ELISA ) technique was applied to detect the HE4 level in the serum of the NC, EnHy and EnCa group. Re-sults: The expression of HE4 protein was higher in the EnCa group than in the NC and EnHy group, with statistical differences amongthe three groups ( P < 0.001 ). The median IOD of HE4 protein was lower in the group with stage Ⅰ endometrial carcinoma than in thegroup with the stage-Ⅱ and Ⅲ carcinoma. The differences in positive IOD expression among the three groups were statistically signifi-cant ( P < 0.001 ). The IOD of HE4 in endometrial carcinoma tissue was positively correlated with the FIGO staging ( P = 0.001 ). Thepreoperative median level of HE4 was significantly higher in EnCa group than in the NC and EnHy group ( P < 0.05 ). In EnCa group,the postoperative level of HE4 was significantly lower in the serum of EnCa group than the preoperative level ( P < 0.05 ). In endometri-al cancer group, the expression of HE4 protein in tissue was positively correlated with the preoperative serum HE4 levels. The preopera-tive serum HE4 levels were positively correlated with the FIGO staging and the level of CA125 in the serum ( P < 0.05 ). Conclusion:The elevated expression of HE4 can be a biomarker for endometrial carcinoma and can be an objective index for early auxiliary diagno-sis, evaluation of curative effect, and prognosis assessment of the carcinoma.Keywords Endometrial carcinoma; Human epididymis gene product 4 (HE4); Integrated optical density ( IOD ) |
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