| 蒲公英对环磷酰胺致小鼠骨髓细胞突变作用的抑制研究 |
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| 引用本文： | 朱蔚云,庞竹林,梁敏仪,张锦宏. 蒲公英对环磷酰胺致小鼠骨髓细胞突变作用的抑制研究[J]. 癌变．畸变．突变, 2003, 15(3): 164-167. DOI: 10.3969/j.issn.1004-616x.2003.03.011 |
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| 作者姓名： | 朱蔚云 庞竹林 梁敏仪 张锦宏 |
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| 作者单位： | 广州医学院医学遗传学与细胞生物学教研室，广东 广州 510182 |
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| 摘 要： | 目的：研究蒲公英(TM)抗突变作用和对细胞增殖的影响。方法：采用小鼠骨髓淋巴细胞有丝分裂指数试验、染色体畸变试验、微核试验，观察蒲公英水煎液对小鼠骨髓淋巴细胞的遗传损伤效应的影响。结果：①环磷酰胺(CP)单独作用时，能抑制小鼠骨髓淋巴细胞的增殖(P<0.01)；与TM联合作用时，有丝分裂指数明显比阳性对照组提高(P<0.01)。②蒲公英低、中、高剂量组均能明显地抑制由于CP引起的染色体畸变率，其抑制率分别为39.62 ％、58.49 ％、54.72 ％，同时对CP诱发的微核率也有明显的抑制效应；蒲公英水煎液不会导致小鼠骨髓淋巴细胞的染色体畸变和微核率的增加(P> 0.05)。结论：蒲公英本身没有致突变性，能抑制CP诱发的染色体畸变和微核率，促进细胞的增殖能力。
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| 关 键 词： | 蒲公英 环磷酰胺 有丝分裂指数 染色体畸变 微核 |
| 文章编号： | 1004-616X(2003)03-0164-04 |
| 收稿时间： | 2002-11-07 |
| 修稿时间： | 2002-11-07 |
THE EFFECT OF TARAXACUM MONGO TO INHIBIT THE MUTATION IN THE BONE MARROW LYMPHOCYTE OF MICE INDUCED BY CYCLOPHOSPHAMIDE |
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| ZHU Wei-yun,PANG Zhu-lin,LIANG Min-yi,et al. THE EFFECT OF TARAXACUM MONGO TO INHIBIT THE MUTATION IN THE BONE MARROW LYMPHOCYTE OF MICE INDUCED BY CYCLOPHOSPHAMIDE[J]. Carcinogenesis,Teratogenesis and Mutagenesis, 2003, 15(3): 164-167. DOI: 10.3969/j.issn.1004-616x.2003.03.011 |
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| Authors: | ZHU Wei-yun PANG Zhu-lin LIANG Min-yi et al |
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| Affiliation: | Department of Medical Genetics and Cell Biology, Guangzhou Medical College, Guangzhou 510182,China |
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| Abstract: | Purpose:To study the effects of Taraxacum mongo(TM) on anti-mutation and cell proliferation. Methods: Mitotic Index(MI), chromosome aberration and micronucleus test in bone marrow lymphocytes of mice were determined. Results: ①The MI of bone marrow lymphocytes in mice were in hibited by CP(P < 0.01),but MI were significantly increased compared with control group after TM oral feeding on mice (P < 0.01). ②The inhibition rates of chromosome aberration were 39.62 ％, 58.49 ％ and 54.72 ％ ,respectively by TM in different concentrations of low, medium and high, furthermore the frequencies of micronucleus(MN) were inhibited by CP(P > 0.05). Conclusion: No mutagenicity was observed on TM .but TM possesses the ability of anti - mutation,and promotes cell proliferation. |
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| Keywords: | Taraxacum mongo cyclophosphamide mitotic index chromosome aberration micronucleus |
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