Monoclonal antibodies to Nocardia asteroides and Nocardia brasiliensis antigens. |
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| Authors: | T Jim nez, A M Dí az, H Zlotnik |
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| Affiliation: | Department of Microbiology and Medical Zoology, School of Medicine, University of Puerto Rico, San Juan 00936. |
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| Abstract: | Nocardia asteroides and Nocardia brasiliensis whole-cell extracts were used as antigens to generate monoclonal antibodies (MAbs). Six stable hybrid cell lines secreting anti-Nocardia spp. MAbs were obtained. These were characterized by enzyme-linked immunosorbent assay, Western blot (immunoblot), and immunofluorescence assay. Although all the MAbs exhibited different degrees of cross-reactivity with N. asteroides and N. brasiliensis antigens as well as with culture-filtrate antigens from Mycobacteria spp., they have the potential for use as reagents in the purification of Nocardia antigens. |
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