中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (50): 9425-9427.doi: 10.3969/j.issn.1673-8225.2011.50.028

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

小鼠热休克转录因子1真核表达载体的构建

欧阳华伟1，谭  军1，李高峰1，罗成群2   

  1. 1湖南省人民医院整形激光美容外科，湖南省长沙市  410005
    2中南大学湘雅三医院烧伤整形外科，湖南省长沙市410013
  • 收稿日期:2011-10-17 修回日期:2011-11-07 出版日期:2011-12-10 发布日期:2011-12-10
  • 通讯作者: 罗成群，教授，博士生导师，主任医师，中南大学湘雅三医院烧伤整形外科。 LCQ6666@163.com
  • 作者简介:欧阳华伟☆，男，1980年生，湖南省郴州市人，2010年中南大学毕业，博士，医师。benchi2008165@sina.com
  • 基金资助:

    国家自然科学基金项目(30672035)。项目名称：从HSF1水平探讨烧伤炎症反应中细胞内源性分子保护机制。

Construction of a eukaryotic expression vector containing mouse heat shock transcription factor 1

Ouyang Hua-wei1, Tan Jun1, Li Gao-feng1, Luo Cheng-qun2   

  1. 1Department of Plastic and Laser Cosmetic Surgery, People’s Hospital of Hunan Province, Changsha  410005, Hunan Province, China
    2Department of Burn and Plastic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China
  • Received:2011-10-17 Revised:2011-11-07 Online:2011-12-10 Published:2011-12-10
  • Contact: Luo Cheng-qun, Professor, Doctoral supervisor, Chief physician, Department of Burn and Plastic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China LCQ6666@163.com
  • About author:Ouyang Hua-wei☆, Doctor, Physician, Department of Plastic and Laser Cosmetic Surgery, People’s Hospital of Hunan Province, Changsha 410005, Hunan Province, China benchi2008165@sina.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30672035*

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被引次数

1

摘要：

背景：研究表明，热休克转录因子1具有热休克应答效应。
目的：构建小鼠热休克转录因子1(heat shock transcription factor 1，HSF1)真核表达载体。
方法：克隆小鼠HSF1 cDNA，将其插入载体pcDNA3.1，构建热休克转录因子1真核表达载体pcDNA3.1-HSF1。
结果与结论：构建的pcDNA3.1-HSF1重组体双酶切电泳后出现大小约5.5 kb与1.5 kb的2个片段，测序结果显示克隆的小鼠HSF1序列与Genbank中的一致，采用Western blot法可检测到一条相对分子质量约72 000的重组HSF1蛋白条带，说明成功构建小鼠HSF1真核表达载体pcDNA3.1-HSF1。

关键词: 热休克转录因子1, 真核表达载体, 基因重组, 小鼠, 组织工程

Abstract:

BACKGROUND: Heat shock transcription factor 1 has heat shock response effect.
OBIECTIVE: To construct a eukaryotic expression vector containing mouse heat shock transcription factor 1 (HSF1).
METHODS: Amplified mouse HSF1 cDNA fragment was cloned into pcDNA3.1 to construct a eukaryotic expression vector pcDNA3.1-HSF1.
RESULTS AND CONCLUSION: There were two fragments in electrophoresis after restriction enzyme digestion, 5.5 kb fragment and 1.5 kb fragment. The result of DNA sequencing showed that the sequence of the cloned HSF1 was identical to that GeneBank had reported. HSF1 protein band with the relative molecular mass of 72 000 was detected by western blot. These findings indicate that the eukaryotic expression vector pcDNA3.1-HSF1 containing mouse HSF1 is successfully constructed.

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