Molecular epidemiology of hand,foot and mouth disease in Tianjin
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摘要: 目的 分析天津市手足口病病原学分布及肠道病毒71型(EV71)分子流行病学特点。方法 采集天津市各医院2008—2010年手足口病住院患者粪便标本2 377份,采用逆转录-聚合酶链反应(RT-PCR)检测肠道病毒核酸;选取部分EV71阳性标本分离病毒,采用RT-PCR扩增其VP1基因,进行序列测定和同源性分析,建立系统发生树。结果 2 377份手足口病粪便标本中,肠道病毒总阳性率为70.72% (1 681/2 377),其中EV71占48.30%,CV-A16占37.36%,其他EV占14.34%;31株EV71分离株与C4亚型的C4a分支参比株具有最高核苷酸序列同源性,同源性为95.7%~99.2%,在系统发生树上均归属于C4a分支。结论 天津市2008—2010年手足口病主要病原体为EV71,其流行株为C4亚型中的C4a病毒株。Abstract: Objective To analyze the pathogens of hand,foot,and mouth disease(HFMD)and the molecular epidemiology of enterovirus 71(EV71)in Tianjin. Methods A total of 2 377 clinical specimens from HFMD patients in Tianjin,2008-2010,were tested by real-time reverse transcription-PCR(RT-PCR).EV71 strains were isolated from some EV71-positive specimens.VP1 genes of the EV71 isolates were amplified by RT-PCR and sequenced.Phylogenetic tree was constructed after homology analysis of VP1 genes. Results Total positive rate of pan-enteroviruses was 70.72% (1 681/2 377)in the HFMD cases.Among the cases,48.30% were positive for EV71,37.36% for CV-A16,and 14.34% for other enterovirus cases.The thirty-one EV71 isolates had the highest homology(95.7%-99.2%)with the reference strain of C4a cluster of the C4 subgenotype.Phylogenetic analysis of entire VP1 gene of the EV71 isolates showed that they belong to C4a cluster of the C4 subgenotype. Conclusion EV71 was the principal pathogen of HFMD in Tianjin,2008-2010.The epidemic strains were C4a viruses of the C4 subgenotype.
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Keywords:
- hand,foot,and mouth disease /
- enterovirus 71 /
- genotype
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