SF3B1/FOXM1/JUNB轴调控SOX21表达对宫颈癌细胞生物学行为的影响研究

doi:10.3969/j.issn.1674-2257.2025.01.001

首页 > 按月查看>2025年第1月 >1-5+10. DOI:10.3969/j.issn.1674-2257.2025.01.001

SF3B1/FOXM1/JUNB轴调控SOX21表达对宫颈癌细胞生物学行为的影响研究
DOI:
                        10.3969/j.issn.1674-2257.2025.01.001
                    
作者:
                        高洁;阿依努尔·色义提;谢丽;夏依拉·艾合买提;侯友翔;高洁;阿依努尔·色义提;谢丽;夏依拉·艾合买提;侯友翔;
新疆医科大学附属肿瘤医院妇科放射治疗科(一病区)
在知网中查找
在百度中查找
在本站中查找

                    
作者单位:
作者简介:
通讯作者:
基金项目:国家自然基金地区科学基金项目(No:82260520,No:81860528)；

EffectofSF3B1/FOXM1/JUNBAxisRegulatingSOX21ExpressiononBiologicalBehaviorofCervicalCancerCell

Author:

Ayinuer·Seyiti；Xie Li；Xiayila·Aihemaiti；Hou You xiang
Ayinuer·Seyiti；Xie Li；Xiayila·Aihemaiti；Hou You xiang
DepartmentofGynecologicRadiotherapy (Ward1), AffiliatedCancerHospitalofXinjiangMedicalUniversity,Urumqi830011,China
在知网中查找
在百度中查找
在本站中查找

Affiliation:

Fund Project:

摘要

图/表

访问统计

参考文献

相似文献

引证文献

资源附件

摘要:

目的分析剪接因子3B亚基1/叉头框转录因子M1/转录因子活化蛋白激酶B(SF3B1/FOXM1/JUNB)轴调控转录因子21抗体(SOX21)表达对宫颈癌细胞生物学行为的影响。方法选取2022年3月至2023年12月新疆医科大学附属肿瘤医院收治的50例宫颈癌患者的癌旁组织及癌组织作为研究对象，利用实时荧光定量PCR检测SF3B1、FOXM1、JUNB、SOX21表达；通过Transwell、细胞计数试剂8(CCK8)检测宫颈癌细胞生物学行为(增殖、迁移、侵袭);利用蛋白质印迹法测定SF3B1、FOXM1、JUNB、SOX21蛋白表达。结果与癌旁组织相比，宫颈癌组织JUNB表达低，FOXM1、SOX21、SF3B1表达高，差异有统计学意义(P<0.05);与si-NC组相比，si-SF3B1/FOXM1/JUNB组0 h OD450值高，侵袭细胞数、迁移细胞数、(24、48 h)OD450值、SF3B1、FOXM1、JUNB低，差异有统计学意义(P<0.05);与OE-NC组相比，OE-SOX21组迁移细胞数、(0、24、48 h)OD450值、SOX21、侵袭细胞数高，差异有统计学意义(P<0.05);与si-SF3B1/FOXM1/JUNB+OE-NC组相比，si-SF3B1/FOXM1/JUNB+OE-SOX21组SOX21、SF3B1、FOXM1、JUNB、(0、24、48 h)OD450值、侵袭细胞数、迁移细胞数高，差异有统计学意义(P<0.05)。结论 SF3B1/FOXM1/JUNB轴通过激活SOX21表达可促进宫颈癌细胞侵袭、增殖、迁移。

关键词:剪接因子3B亚基1/叉头框转录因子M1/转录因子活化蛋白激酶B;转录因子21抗体;宫颈癌细胞;迁移;侵袭;增殖;

Abstract:

Objective Toanalyzetheeffectofsplicingfactor3Bsubunit1/forkheadbox M1/transcriptionfactor activatedproteinkinaseB(SF3B1/FOXM1/JUNB)axisregulatedexpressionoftranscriptionfactor21antibody(SOX21)on thebiologicalbehaviorofcervicalcancercells.Methods Thepara-canceroustissuesandcanceroustissuesof50cervical cancerpatientsadmittedtotheAffiliatedCancerHospitalofXinjiangMedicalUniversityfrom March2022toDecember2023 wereselectedasthestudyobjects.TheexpressionlevelsofSF3B1,FOXM1,JUNBandSOX21weredetectedbyreal-time fluorescencequantitativePCR.Thebiologicalbehaviorsofcervicalcancercells(proliferation,migrationandinvasion)were detectedbyTranswellandcellcountingkit-8(CCK8).TheproteinexpressionsofSF3B1,FOXM1,JUNBandSOX21were determinedby Westernblot.Results Compared withtheadjacenttissues,thecervicalcancertissuesshowedalower expressionlevelofJUNBandhigherexpressionlevelsofFOXM1,SOX21,andSF3B1,andthedifferenceswerestatistically significant(P <0.05).Compared withthesi-NCgroup,thesi-SF3B1/FOXM1/JUNBgroupexhibitedahigheroptical density(OD)450valueat0h,butlowerinvasivecellcount,migratorycellcount,(24,48h)OD450values,SF3B1, FOXM1,andJUNB,andthedifferenceswerestatisticallysignificant(P<0.05).ComparedwiththeOE-NCgroup,the OE-SOX21groupshowedhighermigratorycellcount,(0,24,48h)OD450values,SOX21,andinvasivecellcount,andthe differenceswerestatisticallysignificant (P <0.05).Compared withthesi-SF3B1/FOXM1/JUNB+ OE-NCgroup,the si-SF3B1/FOXM1/JUNB+OE-SOX21groupexhibitedhigherlevelsofSOX21,SF3B1,FOXM1,JUNB,(0,24,48h)OD450 values,invasivecellcount,and migratorycellcount,andthedifferences werestatisticallysignificant (P <0.05).Conclusion SF3B1/FOXM1/JUNBaxiscanpromotetheinvasion,proliferationand migrationofcervicalcancercellsby activatingSOX21expression

Key words:Splicingfactor3Bsubunit1/forkheadboxM1/transcriptionfactoractivatedproteinkinaseB;Antibodyto transcriptionfactor21;Cervicalcancercell;Migration;Invasion;Proliferation

引用本文

高洁;阿依努尔·色义提;谢丽;夏依拉·艾合买提;侯友翔;. SF3B1/FOXM1/JUNB轴调控SOX21表达对宫颈癌细胞生物学行为的影响研究[J].成都医学院学报,2025,20(1):1-5+10

复制

文章指标

点击次数:
下载次数:

历史

收稿日期:
最后修改日期:
录用日期:
在线发布日期: 2025-02-15

引用本文

分享

文章指标

历史