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人脐血间充质干细胞静脉移植治疗大鼠局灶性脑缺血的实验研究
引用本文:陈晓岚,黄仁彬,汤银娟,莫艳秀,刘科峰,刘运海.人脐血间充质干细胞静脉移植治疗大鼠局灶性脑缺血的实验研究[J].中国实用神经疾病杂志,2007,10(1):4-7.
作者姓名:陈晓岚  黄仁彬  汤银娟  莫艳秀  刘科峰  刘运海
作者单位:1. 湖南学院组织学与胚胎学教研室,郴州,423000
2. 湖南郴州市第一人民医院神经内科,郴州,423000
3. 中南大学湘雅医院神经内科,长沙,410008
基金项目:湖南省卫生厅科研基金资助课题(批准号B2005-117)
摘    要:目的 探讨来源于人脐血的间充质干细胞经静脉移植治疗大鼠局灶性脑缺血的可行性及其机制.方法 将人脐血间充质干细胞在体外纯化、扩增并经BrdU标记后,经尾静脉移植到局灶性脑缺血大鼠体内,通过神经缺损评分观察移植后大鼠神经行为学改善情况,通过组织学方法观察移植到脑内的人脐血间充质干细胞表达脑源性神经营养因子和缺血灶周围微血管密度变化的情况.结果 人脐血间充质干细胞移植组大鼠的神经缺损评分显著低于对照组(P<0.05);移植到脑内的人脐血间充质干细胞主要选择性分布于缺血灶周围区域并表达脑源性神经营养因子,移植组大鼠梗死灶周围的微血管密度显著高于对照组(P<0.01).结论 经静脉注射移植人脐血间充质干细胞能明显促进局灶性脑缺血大鼠的神经行为功能恢复,促进缺血灶周边区微血管增生可能是人脐血间充质干细胞移植治疗局灶性脑缺血的机制之一.

关 键 词:人脐血  间充质干细胞  局灶性脑缺血  脑源性神经营养因子  细胞移植  微血管密度
文章编号:1673-5110(2007)01-0004-04
修稿时间:2006-10-24

Study on the therapeutic effects of the intravenous administration of human umbilical cord blood mesenchymal stem cells for treating focal cerebral ischemia in rats
Chen Xiaolan , Huang Renbin , Tang Yinjuan ,et al..Study on the therapeutic effects of the intravenous administration of human umbilical cord blood mesenchymal stem cells for treating focal cerebral ischemia in rats[J].Chinese Journal of Practical Neruous Diseases,2007,10(1):4-7.
Authors:Chen Xiaolan  Huang Renbin  Tang Yinjuan  
Institution:Department of Histology and Embryology, Xiangnan College, Chenzhou 423000
Abstract:Objective To investigate the feasibility and the mechanism of the intravenous administration of human umbilical cord blood mesenchymul stem cells(MSCs)for treating focal cerebral ischemia in rats.Methods After purified,proliferated and marked with BrdU,the MSCs were injected intravenously into rats 7 days after focal cerebral ischemia.Neurological Deficits Score(NDS)were evaluated before and following 1,7,14,21and 28 days of middle cerebral artery occlusion(MCAO).Rats were euthanized at 28 days after MCAO.Brain section were stained with hematoxylin arid eosin(HE)for surveying the volume of infarction.Double staining immunohistochemistry of brain section was used to identify cell-specific brain-derived neurotrophic factor(BDNF)expression in bromodeoxyuridine-reactive MSCs.And microvascular density in ischemic boundary zone was also detected with immunochemical staining of factor.Results NDS in MSCs-transplanted group at 21th day and 28th day of MCAO was significantly lower than that in control group(P<0.05).Double immunostaining showed that grafted MSCs had migrated to the ischemic boundary zone,survived and expressed BDNF.The microvascular density was higher in the ischemic boundary zone of MSCs-transplanted group than that in the control group(P<0.01).Conclusion The intravenous administration of MSCs can promote the recovery of neurological function of rats with focal cerebral ischemia.The therapeutic effect of MSCs on rats with focal cerebral ischemia may be derived from the angiogenesis in the ischemic boundary zone.
Keywords:Human umbilical cord blood  Mesenchymal stem cells  Focal cerebral ischemia  Brain-derived neurotrophic factor  Cell transnlantation  Microvascular density
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