| 反义Noggin基因对成年大鼠海马内Nestin及GFAP表达的影响 |
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| 引用本文： | 徐海伟,范晓棠.反义Noggin基因对成年大鼠海马内Nestin及GFAP表达的影响[J].中国神经科学杂志,2005,21(5):319-323. |
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| 作者姓名： | 徐海伟 范晓棠 |
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| 作者单位： | 第三军医大学生理学教研室 重庆400038(徐海伟)，第三军医大学神经生物学教研室 重庆400038(范晓棠) |
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| 摘 要： | 目的探讨Noggin基因对成年大鼠海马内Nestin及GFAP表达的影响。方法反义寡核苷酸技术封闭内源性Noggin基因的表达,免疫组化法检测成年大鼠海马内Nestin与GFAP的表达。结果侧脑室连续4d注射Noggin基因的反义寡核苷酸后,可见海马齿状回(dentate gyrus,DG)内Nestin阳性细胞数与GFAP阳性细胞数较对照组显著增加;室下区GFAP阳性细胞数亦明显增加。结论Noggin对成年海马干细胞的分化有重要作用,内源性Noggin基因的表达可使神经干细胞向神经元方向分化。
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| 关 键 词： | 免疫组化 大鼠 Noggin 海马 Nestin 胶质原纤维酸性蛋白 |
| 收稿时间： | 2005-04-08 |
Effect of a antisense oligonucleotide to noggin on the expression of nestin and GFAP in the hippocampus of adult rats |
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| Hai-Wei Xu ,Xiao-Tang Fan.Effect of a antisense oligonucleotide to noggin on the expression of nestin and GFAP in the hippocampus of adult rats[J].Neuroscience Bulletin,2005,21(5):319-323. |
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| Authors: | Hai-Wei Xu Xiao-Tang Fan |
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| Abstract: | ObjectiveTo examine the role of noggin on the expression of nestin and glial fibrillary acidic protein (GFAP) in the hippocampus of adult rats. Methods Antisense oligodeoxynucleotide (ASODN) technique was employed to inhibit endogenous noggin expression and immunohistochemistry was used to detect the expressions of Nestin and GFAP in the hippocampus of adult rats. ResultsIt was observed that the number of nestin and GFAP immunoreactive cells in the dentate gyrus (DG) of hippocampus was increased in adult rats treated with antisense oligodeoxynucleotide to noggin. Moreover, the number of GFAP immunoreactive cells was increased in the subventricular zone of the rats treated with antisense oligodeoxynucleotide to noggin. Conclusion The results in the present study indicates that noggin may play a role in the differentiation of neural stem cells in the adult hippocampus, and it promotes the differentiation of neural stem cells in the DG to neuronal fate. |
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| Keywords: | immunohistochemistry rat noggin hippocampus nestin GFAP |
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