针刺对缺血性脑损伤大鼠的治疗作用及对GDNF表达水平和MAPKs信号转导通路的调节

目的研究针刺对局灶性缺血脑损伤大鼠GDNF表达水平和对pERK1／2及pElk-1水平的调节作用。方法采用线栓法建立大鼠大脑中动脉阻断（MCAO）模型，分为针刺组和缺血组，并对前者进行针刺治疗。24h后进行神经功能评分判断疗效，48h后进行TTC染色判断梗塞体积，并采用免疫组织化学和Western-blot法定位定量检测脑组织中GDNF表达水平及信号转导通路蛋白ERK1／2和ELK1磷酸化水平的变化。结果针刺可促进缺血性损伤大鼠神经功能的恢复，并减小脑梗塞体积（P〈0．05）。缺血灶周围区脑组织GDNF、pERK1／2及pElk1的含量均增高。针刺组pERK1／2及pElk1的含量低于缺血组（P〈0．01）；而GDNF的含量高于缺血组（P〈0．01）。结论针刺对缺血性脑损伤大鼠有保护作用，其作用机制可能涉及良性调节MAPK信号通路的反应性，上调GDNF的表达。

Effect of acupuncture on expression of GDNF and activation of MAPKs in the rat brain after focal cerebral ischemic injury

Objective To investigate the effect of acupuncture on the expression of GDNF and the phosphorylation of ERK1/2 and Elk-1 in the rat brain after focal cerebral ischemic injury. Methods A model of middle cerebral artery occlusion was established by intraluminal suture in adult male SD rats. Acupuncture treatment was applied daffy. Animals were evaluated neurologically at 24 h after reperfusion, and decapitated at 48 h for TTC staining, immunohistochemistry and Western-blot analysis. Results Rats that had undergone acupuncture treatment had a better functional recovery with a smaller infarction size. Meanwhile, the expression of GDNF in the brain increased significantly whereas the expression of p-ERK1/2 and p-Elk1 decreased after acupuncture treatment. Conclusion Acupuncture seems to protect rats from cerebral ischemic injury by regulating MAPK signal transduction and promoting GDNF expression.