Intramembrane particle distribution and lectin binding of glioblastoma cells after long term subculture |
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Authors: | James C Hogan Luara Manuelidis |
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Institution: | (1) Department of Pathology, Yale University School of Medicine, 310 Cedar Street, 06510 New Haven, Connecticut, USA |
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Abstract: | Summary Human glioblastoma cells in long-term monolayer culture showed an even distribution of intramembrane particles (IMP) on all surfaces of the plasma membrane; junctional complexes were rearely observed and rectilinear arrays were not seen. Cells treated with Con A-ferritin an dRicin II-ferritin showed an even distribution of lectin receptors and under conditions used no capping occurred. Lectin-ferritin complexes were taken up into pinocytotic vesicles. Cleaved preparations of Ricin II-ferritin treated cells showed no change in the distribution of IMP.The authors wish to thank Dr. E. E. Manuelidis for invaluable advice, J. P. Albert and P. Johnson for expert technical assistance, and Mrs. M. Silberberg for excellent secretarial work.This work was supported by NIH Grants GM 02140-05 and CA 15044-03 and USPHS 5 P01 NS-06208-11. |
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Keywords: | Glioblastoma Tissue culture Freeze-fracture Intramembrane particles Ricin II-ferritin Con A-ferritin |
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