| 不同放疗敏感性胶质瘤组织蛋白质组学比较研究 |
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| 引用本文： | 阎华,胡新华,肖红,邹元杰,章文斌,刘宏毅.不同放疗敏感性胶质瘤组织蛋白质组学比较研究[J].临床神经外科杂志,2011,8(1):4-6. |
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| 作者姓名： | 阎华 胡新华 肖红 邹元杰 章文斌 刘宏毅 |
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| 作者单位： | 1. 南京医科大学附属脑科医院神经外科,210029
2. 南京医科大学附属脑科医院研究所,210029 |
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| 摘 要： | 目的 比较放疗敏感性不同胶质瘤间蛋白质组差异,寻找可成为胶质瘤放射敏感性标记物的蛋白质.方法 收集放疗前的胶质瘤组织标本,以放疗后预后为判断标准,选择不同放疗敏感标本各3例,分为敏感组和耐受组.用二维液相色谱-质谱联用方法对肿瘤蛋白质组进行分析,筛选两组之问表达差异蛋白.应用Western印迹技术验证差异蛋白的表达.结果 丝切蛋白-1在放疗耐受组中表达明显增高,胶质瘤细胞大体形态无变化.结论 丝切蛋白-1可能与胶质瘤放疗敏感性相关.
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| 关 键 词： | 胶质瘤 放射治疗 蛋白质组学 差异表达蛋白 |
Comparative study on the proteomic analysis in glioma tissues with different radiosensitivities |
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| Institution: | YAN Hua, HU Xin-hua, XIAO Hong, et al. Department of Neurosurgery, Brain Hospital Affiliated to Nanjing Medical University, 210029 Nanjing, China |
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| Abstract: | Objective To gain a better understanding of prognosis of radioresistant gilomas, therefore a critical need to develop a new system that more precise and objective to evaluate the patients＇ prognosis. Methods Six typical patients were chosen and the proteome patterns of their gliomas were analyzed with two-dimensional liquid chromatography tandem mass spectrometry （2D- LC-MS/MS）. The results of the proteome-based analysis for a subset of proteins related to radioresistant was confirmed by western blot. Results The present study showed that cofilin-1 was overexpression in radioresistant gliomas but not with the gross morphology change. Conclusion Cofilin-1 may be the potential predictive marker for radiosensitivity in gliomas. |
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| Keywords: | glioma radiotherapy proteomics differentially expressed proteins |
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