凋亡调控基因在难治性颞叶癫痫患者脑中表达的研究

目的:探讨神经元凋亡与难治性癫痫患者海马硬化关系。方法:16例颞叶癫痫患者手术标本,用光镜、电镜及原位末端标记(TUNEL)方法检测神经元凋亡;应用免疫组化方法检测Bax、Bcl-2蛋白的表达。结果:对照组未发现凋亡神经元;癫痫组在电镜检查的3例标本中,1例有少量早期凋亡征象。对照组Bcl-2蛋白无表达,癫痫组Bcl-2蛋白表达明显增强(P<0.001);Bax蛋白在癫痫组与对照组中均微弱表达,两者差异无统计学意义(p>0.5)。结论:凋亡基因Bcl-2、Bax参与癫痫过程。

Study on expressions of apoptosis regulatory genes in brains of patients with medically intractable temporal lobe epilepsy

Objective:To explore the relation between neuron apoptosis and hippocampus sclerosis of patients medically intractable temporal lobe epilepsy.Methods:The specimens were obtained from 16 patients with temporal lobe epilepsy,investigated neuron apoptosis by light microscopy,electron microscopy and TdT-mediated dUTP-biotin nick end labeling(TUNEL)method,and determined the expression of Bcl-2 and Bax by immunohistochemistry.Results:The evidence of neuron apoptosis was not found by those ways.But some early signs of neuron apoptosis in 1 patients(1/3)were found by electron microscopy in epilepsy group.Unlike that in normal adult brain,Bcl-2 immunoreactivity was obviously observed in some neurons in epilepsy group(P<0.001)compared with normal group,Bax immunoreactivity was not increased in epilepsy group(P>0.5).Conclusion:Apoptosis regulatory genes Bcl-2 and Bax relate to the pathogenesis of epilepsy.