| GABA对体外培养大鼠神经元GABA_A受体亚单位mRNA表达的影响 |
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| 引用本文： | 李晓青,刘鹏,种莉,唐鹏,刘玥,陈丽,张欣,侯辰,李锐,郭民侠.GABA对体外培养大鼠神经元GABA_A受体亚单位mRNA表达的影响[J].脑与神经疾病杂志,2014(4):260-263. |
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| 作者姓名： | 李晓青 刘鹏 种莉 唐鹏 刘玥 陈丽 张欣 侯辰 李锐 郭民侠 |
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| 作者单位： | 陕西省人民医院神经内三科,西安710068 |
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| 基金项目： | 陕西省重点领域科技创新团队项目（2012KCT-17） |
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| 摘 要： | 目的观察γ-氨基丁酸(GABA)对体外大鼠培养神经元GABAA受体亚单位mRNA表达的影响,探讨肝性脑病(HE)发病机制。方法采用原位杂交技术,观察GABA对体外培养新生大鼠神经元GABAA受体亚单位α1β1γ2mRNA表达的改变。结果与对照组相比,GABA组神经元GABAA受体亚单位α1及γ2(增幅14%,P<0.01)的mRNA表达显著上升,亚单位β1的mRNA表达显著减少(降幅11%,P<0.05)。结论GABA可通过影响GABAA受体亚单位的表达发挥其对肝性脑病的致病作用。
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| 关 键 词： | γ-氨基丁酸 神经元培养 原位杂交 GABAA受体 |
Effect of GABA on the e xpression of the mRNA of GABAA receptor subunits in cultured rat neurons |
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| LI Xiao-qing,LIU Peng,CH ONG Li,TANG Peng,LIU Yue,CHEN Li,ZHANG Xin,HOU Chen,LI Rui,GUO Min-xia.Effect of GABA on the e xpression of the mRNA of GABAA receptor subunits in cultured rat neurons[J].Journal of Brain and Nervous Diseases,2014(4):260-263. |
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| Authors: | LI Xiao-qing LIU Peng CH ONG Li TANG Peng LIU Yue CHEN Li ZHANG Xin HOU Chen LI Rui GUO Min-xia |
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| Institution: | (Department of Neurology, the Shanxi Provincial People＇s Hospital, Xian 710068, China) |
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| Abstract: | Object ive To observe the expression of gama-aminobutyric acid A （ GABAA ） receptor subunits mRNA in vitro cultured rat neurons by gama-aminobutyric acid （ GABA ） , discuss the pathogenesis of hepatic encephalopathy （ HE） .Methods Using the hybridization in situ to observe the change of GABA A receptor subunitsα1 ,β1 and γ2 mRNA in vitro cultured rat neurons by GABA .Results In group of cultured neurons by GABA , mRNA expression levels of GABA A receptor subunits α1 ,γ2 （ increasing amplitude 14%, P〈0.05 ） increased significantly than those of control group but subunit β1（ decreasing amplitude 11%,P〈0.05） decreased significantly . Conclusion The effect of GABA on the HE could be conducted by modulating the expression of the GABA A receptor subnuit . |
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| Keywords: | Gama-aminobutyric acid Neuron culture Hybridization in situ Gama-aminobutyric acid A receptor |
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