脑卒中患者血管紧张素转换酶基因多态性分析

目的：研究血管紧张素转换酶（ＡＣＥ）基因多态性和脑卒中的发病关系。方法：应用ＰＣＲ方法检测１０５例脑卒中患者、１０６例原发性高血压（ＥＨＴ）患者及６４例正常人的 ＡＣＥ基因的缺失／插人（Ｄ／Ｉ）多态性。结果：脑卒中组ＡＣＥ基因型及等位基因频率与ＥＨＴ组及正常对照组比较差异无显著意义，脑卒中组中脑梗死组和脑出血组分别与正常对照组比较ＡＣＥ基因型均无显著差异。脑卒中伴ＥＨＴ组与无伴ＥＨＴ组比较，Ⅱ基因型频率明显升高，而ＤＤ基因型频率则明显降低；脑卒中伴 ＥＨＴ组Ⅱ基因型频率明显高于 ＥＨＴ组，而 ＤＤ基因型频率远低于 ＥＨＴ组和正常对照组，同时脑卒中伴ＥＨＴ组的平均年龄较其它组明显高。结论：原发性高血压患者中Ⅱ基因型携带者可能具有卒中易感性，ＤＤ基因型携带者有随年龄增加而减少的趋势。

Analysis of angiotensin converting enzyme gene ploymorphism in patiens with stroke

Objective： The purpose of the study was to investigate the relationship between the polymorphism of the angiotensin converting enzyme (ACE) gene and the coming on of stroke.Methods: An iinsertion/deletion polymorphism of the ACE gene was analysed by polymerase chain reaction was determined in 105 cases of stroke, 106 cases of essential hypertension (EHT) and 64 control subjects. Results: No differerce in genotype and allele gene frequency was observed beteen the patients with stroke and the control subjects or between the control subjects and the patients with cerebral infarction or with cerebral hemorrhage (P>0.05). The frequency of the Ⅱ genotype of the ACE gene was significantly higher in the stroke patients with EHT than in those without EHT (χ2=4.08， P<0.05), yet the fequency of the DD genotype was on the contrary (χ2=8.85， P<0.01). The frequency of the Ⅱ genotype of the ACE gene was significantly higher in the stroke patients with EHT than in the EHT patientswithout stroke (χ2=7.24， P<0.01)， yet the frequency of the DD genotype was obviously lower in the stroke patients wiht EHT than the EHT patients and the control subjects (χ2=5.30 and 4.69， P<0.05). No difference was observed between the EHT patients and the control subjects. The average age of the stroke patients with EHT was significantly higher than the EHT patients and the cotrol subjects. (t=4.62 and 7.07， P<0.001)Conclusion: These suggested that the Ⅱ genotype of ACE gene increased risk stoke in EHT patients. Besides, there was a significant decline in the frequency of DD genotype wiht increasing age.