降钙素基因相关肽及内皮素在实验性蛛网膜下腔出血后急性与迟发性脑血管痉挛中的作用

目的　探讨降钙素基因相关肽 (CCRP)及内皮素 (ET)在实验性蛛网膜下腔出血 (SAH)后急性及迟发性脑血管痉挛 (CVS)过程中的作用。方法　采用枕大池穿刺一次注血法 (0 .5ml/kg)制成兔SAH动物模型。运用经颅多普勒 (TCD)观察兔基底动脉 (BA)痉挛情况。在不同时间点运用放射免疫技术定量测定脑脊液中CGRP及ET浓度变化。结果　兔SAH后 30分钟、3天、5天TCD检查显示 ,兔基底动脉处于痉挛状态。脑脊液中CGRP浓度在SAH后 30分即明显上升达术前 18倍 (P <0 .0 5 ) ,第 3、5天浓度明显回落 ,但仍然高于术前 (均P <0 .0 5 )。ET浓度在SAH后 30分钟时无明显改变 ,第 3、5天增高达术前的 2倍 (均P <0 .0 5 )。结论　CGRP及ET在急性和迟发性CVS中的作用不同。CGRP对急性CVS有一定的缓解作用 ,CGRP的分泌下降及ET的过度分泌可能参与迟发性CVS的发生及发展过程

The effect of calcitonic gene-related peptide and endothelin on acute and delayed cerebral vasospasm in experimental subarachnoid hemorrhage

Objective To evaluate the effect of calcitonic gene related peptide(CGRP) and endothelin(ET) on acute and delayed cerebral vasospasm(CVS) after subarachnoid hemorrhage(SAH).Methods The SAH model in rabbit were established by single injection of autologous arterial blood(0.5 ml/kg) to cisterna magna on percutaneous suboccpital route. Transcranial Dopper(TCD) was performed to detect the spastic of rabbit basilar artery(BA). The CGRP and ET concentrations in cerebrospinal fluid were examinated quntitatively by radioimmunologlc techniques at 30 minutes, 3 days, and 5 days after SAH.Results The results of TCD showed that rabbit basilar artery was spastic at 30 minutes, 3 days and 5 days after SAH. At 30 minutes after SAH, CGRP concentration significantly increased upto about 18 fold( P <0.05). At 3 days and 5 days after SAH,CGRP concentration significantly returned toward base level but remained higher than control(all P <0.05). ET concentration showed no increasing at 30 minutes, but significantly raised at 3 days,5 days about 2 fold(all P <0.05).Conclusion The effect of CGRP and ET on acute and delayed CVS are different. CGRP may remit the acute CVS to some extents. The deficiency of CGRP and over secreting of ET may paly a role in the occurance and development of the delayed CVS paoceses.