铅暴露鼠学习记忆障碍相关机制的研究

目的 研究铅暴露大鼠海马神经细胞精氨酸加压素(AVP)、生长抑素(SS)的阳性表达,及其与学习记忆障碍的关系.方法 将健康两月龄SD大鼠60只,随机分为对照组、染铅组,每组30只,染铅动物饮用0.1%醋酸铅去离子水3个月,制成慢性染铅大鼠模型;对照组饮用去离子水.Y-迷宫试验检测两组大鼠的学习和记忆能力,采用免疫组化方法测定大鼠海马AVP和SS阳性神经元数,观察铅暴露对其阳性表达的影响.结果 染铅组大鼠学习记忆能力较对照组明显减低(P<0.01),海马CA1区AVP 和SS阳性神经元数量均明显减少(均P<0.01),CA3区AVP(P<0.05)和SS(P>0.05)阳性神经元数量亦减少,但不如CA1区明显.结论 铅暴露大鼠学习记忆能力的减低可能与海马神经细胞AVP和SS的阳性表达减少有关.

Study of relative mechanism of the disorder of learning and memory in lead exposure rats

Objective To investigate the relationship between the disorder of learning and memory in lead rats and the argnine vasopressin (AVP) and the somatotatin (SS) of hippocampus.Methods Sixty healthy 2-month old Sprague-Dawley rats were randomly divided into normal control group and lead group. Normal control group was given deionized water and the lead group was given 0.1% lead acetate deionized water for 3 months to make chronic lead rat. Learning and memory ability was tested by the Y-maze test. The content of AVP and SS in hippocampus were tested by the immunohistochemical method. Results The learning memory ability and the content of AVP and SS in hippocampus CA1 section of the lead group were degraded obviously than the control group(all P<0.01). The content of AVP(P<0.05)and SS(P>0.05)in hippocampus CA3 section of the lead group were degraded also.Conclusion The disorder of the learning and memory of the lead exposure rats may be due to the decrease of the content of AVP and SS in hippocampus.