神经生长因子对新生大鼠缺氧／缺血性脑损伤远期智能的影响

目的 探讨神经生长因子（NGF）对新生大鼠缺氧／缺血性脑损伤（HIBD）后远期智能的影响。方法 建立新生大鼠缺氧／缺血性脑损伤模型，分别给予NGF0．01mg/10g（小剂量组）和0．03mg/10g（大剂量组）腹腔注射，有用迷宫实验观察大鼠远期学习记忆能力的改变，用高效液相色谱电化学测定单胺类递质的改变。结果 NGF组（小剂量组、大剂量组）学习记忆功能明显好于对照组，表现在达标所需反映次数明显低于对照组（P＜0．05），而正确率却明显高于对照组（P＜0．05）；小剂量NGF组与大剂量NGF组相比上述指标的改变也有显著性差异（P＜0．05）；NGF组去甲肾上腺素（NE）、高香草酸（HVA）、5－羟色胺（5－HT）等单胺类递质含量显著低于对照组（P＜0．01）。NGF组脑部改变不明显，仅见轻微的脑皮层萎缩；对照组大鼠脑部可见皮层萎缩、液化及空洞形成，病理组织学可见筛状软化灶形成，胶质结节和钙化灶的形成等。结论 NGF可预防和治疗新生大鼠缺氧／缺血性脑病所致的智能障碍，还可以减轻HIBD时单胺类神经递质的代谢紊乱而促进其智能障碍的恢复。

The effect of long term intelligence on NGF to the newborn rat with hypoxic-ischemic brain damage

Objective To investigate the effect of long term intelligence on NGF to the newborn rats with hypoxic ischemic brain damage (HIBD).Methods The model of newborn rat hypoxic ischemic brain damage(HIBD) was set,0.01mg/10g dosage NGF and 0.03mg/10g dosage NGF were administered to the rat abdominal cavity respectively. With maze test, the change of learning memory abilities of long term in the rats were observed, the concentration of monoamine neurotransmitters in the brain tissue were determined by high performance liquid chromatography electrochemical detector.Results The function of learning and memory was better in NGF group(small, large amount group)than in the control group, the frequency was lower remarkably than the control group( P <0.05),the correct rate was higher than the control group(P< 0.05 );there was remarkable difference in the above index between small dosage of NGF group and large dosage of NGF group( P <0.01);the content of monoamine neurotransmitters especially NE, HVA and 5 HT was lower in NGF group than in the control group( P <0.01).The formation of softening focus, calcific focus and colloid were seen, in the NGF group only mild atrophy of brain layer can be seen, there was no significant change in brain, but in the control group, there was cortical atrophy, liquefaction and cavernous formation.Conclusion NGF could prevent and treat the intelligence defecit following HIBD, and might lighten the disorder of the monoamine neurotransmitters metabolism in HIBD and improved the recovery of its intelligence disorder.