不同剂量巴曲酶对脑缺血沙土鼠的脑保护作用

目的　探讨巴曲酶对脑缺血沙土鼠脑保护作用的最佳剂量。方法　80只沙土鼠随机分为假手术组、对照组、巴曲酶治疗组(又分为1BU/kg、2BU/kg、4BU/kg、8BU/kg、16BU/kg、32BU/kg组),每组10只。制作完全性前脑缺血再灌注沙土鼠模型,制作模型前3h给予对照组沙土鼠腹腔注射生理盐水;巴曲酶组腹腔注射相应剂量的巴曲酶。用原位末端标记(TUNEL)法染色检测沙土鼠海马CA1 区凋亡细胞,光镜下计算海马CA1 区的神经元凋亡率。结果　巴曲酶8BU/kg、16BU/kg、32BU/kg组海马CA1 区细胞凋亡率明显减少,与对照组及巴曲酶1BU/kg、2BU/kg、4BU/kg组比较差异有显著性(均P<0. 01), 8BU/kg、16BU/kg、32BU/kg组之间差异无显著性(均P>0 .05)。结论　巴曲酶具有抗脑缺血后的细胞凋亡作用;巴曲酶8BU/kg为对脑缺血沙土鼠脑保护作用的最佳剂量。

Protective effect of different dose of batroxobin in cerebral ischemic gerbils

Objective To study the best dose of batroxobin that protects forebrain ischemia reperfusion injury in gerbils.Methods 80 gerbils were randomly assigned to receive different dose of batroxobin (1 BU/kg, 2 BU/kg, 4 BU/kg, 8 BU/ kg, 16 U/kg or 32 BU/kg) in treatment group, NS in control group or nothing in sham-operation group. There were 10 gerbils in each group. NS (control group) or batroxobin (treatment group) was given intraperitoneally three hours before establishment of forebrain ischemia reperfusion model. Apoptotic cells were detected by TUNEL technique and the number of apoptotic cells in the hippocampal CA 1 territory was counted using Olympus microscope.Results The apoptotic cells in the treatment groups (8 BU/kg, 16 BU/kg, 32 BU/kg) markedly decreased than those in the control group and the other treatment groups (1 BU/kg, 2 BU/kg, 4 BU/kg) (all P<0.01). There was no statistic difference among high dose of batroxobin treatment groups (8 BU/kg, 16 BU/kg, 32 BU/kg)(all P>0.05). Conclusions Batroxobin has a role of reducing neuron apoptosis after cerebral ischemia. The best dose of batroxobin that protects forebrain ischemia reperfusion injury in gerbils is 8 BU/kg.