碱性成纤维细胞生长因子等体外诱导成人骨髓间充质干细胞分化为神经元样细胞的研究

目的 成人骨髓间充质干细胞(hMSCs)体外定向诱导分化为神经元样细胞。方法 采用Percoll分离液离心分离hMSCs，体外扩增，分别采用含碱性成纤维细胞生长因子(bFGF)和2-巯基乙醇(2-ME)等无血清DMEM诱导hMSCs分化为神经元。免疫组化鉴定神经元烯醇化酶(NSE)、神经丝蛋白(NF)、胶质纤维酸性蛋白(GFAP)。结果 hMSCs在体外扩增传至5代后，流式细胞仪显示99．5％，97．8％，98．8％hMSCs表面抗原CD29、CD44、CD90表达阳性。接种到12孔板，3d后加入bFGF和2-ME联合或2-ME单种诱导剂诱导后，hMSCs胞体收缩，突起伸出；免疫组化显示诱导出的神经元样细胞NSE、NF表达阳性，GFAP阴性。结论 成人骨髓间干细胞在体外可以分化为神经元样细胞。

Human mesenchymal stem cells differentiate into neuron-like cells with bFGF

Objective To investigate the differentiation from human mesenchymal stem cells(hMSCs)into neuron like cells. Methods hMSCs were separated from rib marrow with Percoll reagent and expanded in culture medium. hMSCs were induced to differentiate into neurons with basic fibroblast growth factor (bFGF) and 2 mercaptoethanol(2 ME),respectively.Neuron specific enolase(NSE),neurofilament(NF),nestin,glial fibrillary acidic protein(GFAP) were detected by immunohistochemistry.Results hMSCs were expanded to be undifferentiated cells in culture for more than 5 passages.Flow cytometry showed that 99.5%,97.8%,98.8% hMSCs expressed strongly antigens of CD 29 ,CD 44 ,CD 90 which was identical to human bone marrow derived MSCs.Immunohistochemistry staining showed that the induced different passage hMSCs expressed NSE,NF and nestin,but did not express GFAP.Conclusion It suggests that hMSCs can be differentiated into neurons in vitro.