| 亚低温治疗对脑缺血大鼠AQP4基因表达及血脑屏障通透性的影响 |
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| 引用本文： | 任丽,王玉凯,李红芳,陈芸,郝永楠,徐鹏.亚低温治疗对脑缺血大鼠AQP4基因表达及血脑屏障通透性的影响[J].中风与神经疾病杂志,2011,28(5). |
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| 作者姓名： | 任丽 王玉凯 李红芳 陈芸 郝永楠 徐鹏 |
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| 作者单位： | 1. 济宁医学院附属医院神经内科,山东济宁,272029
2. 中山大学附属佛山医院神经内科,广东佛山,528000 |
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| 摘 要： | 目的 观察亚低温治疗对脑缺血大鼠AQP4表达和血脑屏障通透性的变化的影响,探讨亚低温减轻缺血性脑水肿的可能机制.方法 线栓法制作脑缺血大鼠模型,实验分常温组、亚低温组和假手术组,分别在缺血后6h、24h、48h、72h用干湿重法测脑组织含水量,荧光法检测血脑屏障通透性的改变,原位杂交检测AQP4的表达变化.结果 假手术组脑组织含水量、血脑屏障通透性、AQP4表达无变化.缺血组脑组织含水量和血脑屏障通透性增加,AQP4表达上调,缺血48～72h变化最显著.相同时间点常温组脑组织含水量和血脑屏障通透性增加、AQP4的表达上调比亚低温组明显,两组之间的差异具有统计学意义(P＜0.05).结论 亚低温条件下AQP4表达下调、血脑屏障通透性和脑水肿程度减轻.亚低温可能通过下调AQP4表达、减轻血脑屏障通透性,减轻缺血性脑水肿.
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| 关 键 词： | 亚低温 脑缺血 AQP4 血脑屏障 |
Influence of mild hypothermia on the expression of AQP4 and the permeability of blood-brain barrier after rat cerebral ischemia |
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| REN Li,WANG Yu-kai,LI Hong-fang,et al..Influence of mild hypothermia on the expression of AQP4 and the permeability of blood-brain barrier after rat cerebral ischemia[J].Journal of Apoplexy and Nervous Diseases,2011,28(5). |
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| Authors: | REN Li WANG Yu-kai LI Hong-fang |
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| Institution: | REN Li,WANG Yu-kai,LI Hong-fang,et al.(Department of Neurology Affiliated Hospital of Jining Medical Collage,Jining 272029,China) |
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| Abstract: | Objective To study the influence of mild hypothermia on the expression of AQP4 and the permeability of blood-brain barrier(BBB)after rat cerebral ischemia,and to detect the mechanisms of mild hypothermia that decreases the water content in ischemic brain tissue.Methods Cerebral ischemic models were established by occluding unilateral middle cerebral arteries of rats with threads.The rats were divided into normal temperature(NT)group,mild hypothermic(MHT)group and sham operation group.The brain water content... |
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| Keywords: | Mild hypothermia Cerebral ischemia AQP4 Blood-brain barrier |
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