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| 帕金森病大鼠胃肠功能障碍和肌间神经丛一氧化氮变化的研究 | |
| 引用本文： | 赵静,郭钢花,臧卫东,张华,罗常月,李倩倩.帕金森病大鼠胃肠功能障碍和肌间神经丛一氧化氮变化的研究[J].中风与神经疾病杂志,2011,28(7). |
| 作者姓名： | 赵静 郭钢花 臧卫东 张华 罗常月 李倩倩 |
| 作者单位： | 1. 郑州大学第五附属医院康复科,河南,郑州,450052 2. 郑州大学基础医学院,河南,郑州,450052 3. 商丘市第一人民医院神经内科,河南,商丘,476000 4. 郑州大学第二附属医,神经内科,河南,郑州,450014 |
| 摘 要： | 目的探讨6-羟基多巴胺(6-hydroxydopamine,6-OHDA)诱导的SD大鼠帕金森病(PD)模型胃肠功能障碍及肌间神经丛一氧化氮的变化。方法 60只SD大鼠随机分为对照组和6-OHDA组,每组30只,以6-OHDA诱导制备PD大鼠模型。4w后收集大鼠1h粪便排出量;计算粪便含水量;测定餐后2h大鼠胃内固体食物残留率。采用免疫组化法检测胃肠神经丛神经元型一氧化氮合酶(nNOS)表达变化;反转录聚合酶链式反应技术(RT-PCR)检测胃、结肠组织nNOS mRNA水平的变化。结果与对照组相比,6-OHDA组1h粪便湿重、干重、及含水量明显降低,胃内固体食物残留率明显增加(均P<0.01);胃窦和结肠肌间神经丛nNOS阳性区平均积分光密度明显降低(P<0.01);胃窦、结肠组织nNOS mRNA水平明显降低(P<0.01)。结论 PD大鼠胃肠功能障碍可能与胃肠神经系统nNOS水平降低有关。 |
| 关 键 词： | 帕金森病 肠神经系统 胃肠道 神经元型一氧化氮合酶 |
Gastrointestinal dysfunction and nitric oxide changes of myenteric plexus in a rodent model of Parkinson's disease |
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| ZHAO Jing,GUO Gang-hua,ZANG Wei-dong,et al..Gastrointestinal dysfunction and nitric oxide changes of myenteric plexus in a rodent model of Parkinson's disease[J].Journal of Apoplexy and Nervous Diseases,2011,28(7). | |
| Authors: | ZHAO Jing GUO Gang-hua ZANG Wei-dong |
| Institution: | ZHAO Jing,GUO Gang-hua,ZANG Wei-dong,et al.(Department of Neurology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China) |
| Abstract: | Objective To investigate the gastrointestinal dysfunction and the alteration of nitric oxide in the myenteric plexuses of rat model of Parkinson's disease induced by 6-hydroxydopamine(6-OHDA).Methods Sixty SD rats were randomly divided into two groups:control group and 6-OHDA group,thirty rats each group.Rat model of Parkinson's disease was induced by 6-hydroxydopamine(6-OHDA).Four weeks later,the one-hour fecal output,stool water content and residual solid food in the stomach at 2h after feeding were measu... |
| Keywords: | Parkinson's disease Enteric nervous system Gastrointestinal Neuronal nitric oxide synthase |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |