湖南汉族人群ApoA5基因T-1131C多态性分布及其与脑梗死的关系

目的探讨载脂蛋白A5(ApoA5)基因T-1131C多态位点在湖南地区汉族人群中的分布及其与脑梗死和血脂的关系。方法我们收集200例正常对照组和153例脑梗死患者血标本,用聚合酶链反应-限制性片段长度多态性分析法检测ApoA5基因T-1131C多态性在脑梗死组和正常对照组的基因频率。同时检测研究对象的血脂和脂蛋白水平。结果中国湖南地区人群存在ApoA5基因T1131C多态性,在正常对照组中T/C等位基因频率是0.717/0.283,脑梗死组ApoA5T-1131C等位基因频率显著低于正常对照组,差异有统计学意义(P<0.05),C等位基因携带者的TG,LDL水平显著高于对照组,差异有统计学意义(P<0.05)。结论ApoA5基因多态性可能参与血脂代谢,并与中国湖南汉族人群脑梗死的发生相关。

Relationship between T-1131C Polymorphism of Apolipoprotein A5 and cerebral infarction in Chinese Han population in Hunan

Objective To investigate the relationship between T1131C polymorphism of ApoA5 gene and the occurrence of cerebral infarction(CI) as well as the lipid concentrations in Chinese Han population of Hunan area.Methods 200 healthy individuals and 153 CI patients were recruited in this study.The genotypes of ApoA5 were detected with polymerase chain reaction(PCR) and digested by specific restriction enzymes.Results ApoA5 polymorphism existed in Chinese Han population of Hunan area,with the allele frequencies 0.717/0.283 for T/C in the control group.The frequency of-1131C allele in ApoA5 were significantly lower in CI group compared with that in control group(P<0.05).C allele carriers had a significantly higher level of TG and LDL than subjects with TT genotype.Conclusions ApoA5T-1131C polymorphism might be associated with the development of CI in Chinese Han population of Hunan through a participation in the lipid metabolism.