雷帕霉素对PC12细胞氧糖剥夺损伤的保护作用

目的探讨雷帕霉素对PC12细胞氧糖剥夺损伤(oxygen and glucose deprivation,OGD)的作用。方法选取处于对数生长期的PC12细胞,分为正常组、OGD组、溶剂组和1 nmol、10 nmol、100 nmol、500 nmol雷帕霉素组,OGD同时给予雷帕霉素处理,8 h后通过倒置显微镜观察细胞形态学的变化、MTT法检测细胞存活率的变化以及LDH活性检测细胞受损程度。结果与正常组相比,单纯OGD组细胞活性明显下降,且具有统计学意义(P0.05);与溶剂组相比,1、10、100、500 nmol雷帕霉素组细胞存活率均存在不同程度的增高,其中10、100、500 nmol组具有统计学意义(P0.05)。结论雷帕霉素对PC12细胞OGD损伤具有保护作用。

The effect of rapamycin on PC12 cells injured by oxygen and glucose deprivation

Objective To investigate the effect of rapamycin on PC12 cells injured by oxygen and glucose deprivation.Methods The PC12 cells which in the logarithmic phase were randomly divided into control,OGD,vehicle,1,10,100,500 nmol rapamycin groups.Rapamycin was added to the medium directly at the start of OGD.We observed the morphology changes of PC12 cells by inverted microscope after OGD,evaluated the survival rate of PC12 cells challenged with the MTT assay.Results Compared with the control group,the PC12 cells activity of OGD group was significantly decreased (P<0.05),compared with the vehicle group,the cell survival rates of 1,10,100,500 nmol rapamycin groups were increased in a varying degrees,and cell survival rates of the 10,100,500 nmol have statistically significant (P<0.05).Conclusion Rapamycin has a protective effect in OGD injury of PC12 cells.