二苯乙烯苷对大鼠癫痫引发的学习记忆功能的影响及保护作用

目的：研究二苯乙烯苷（tetrahydroxy stilbene glucoside, TSG）对癫痫大鼠脑损伤的保护作用及可能机制。方法：将SD大鼠随机分为假手术组、模型组、TSG干预组，应用立体定向脑室内微量注射的方法建立海人藻酸（KA）诱导的大鼠癫痫模型。5d后通过Morris水迷宫实验测定大鼠学习记忆功能；取脑行Nissl染色观察大鼠海马神经元的形态及数目改变。结果：与假手术组相比，模型组大鼠学习记忆能力明显降低，海马CA1区神经元大量丢失，结构紊乱。而TSG干预组可使癫痫引发的学习记忆能力降低的情况得到改善，抑制海马CAl区神经元的丢失，改善神经元结构。结论：TSG对癫痫引发的脑损伤，尤其是对海马区神经元迟发性凋亡具有明显的改善作用。

Effect and protection of learning and memory functions by tetrahydroxy stilbene glucoside in hippocampus of epilepsy-rats

Objective：To investigate the protective effect of tetrahydroxy stilbene glucoside （TSG） on learning and memory functions in hippocampus of epilepsy-rats. Methods：Mature male Sprague-Dawley （SD） rats were devided into three groups：Sham Operated group, Model group and TSG group. Seizures were induced by KA through intracerebroventricular injection by a stereotaxic apparatus. The functions of learning and memory were detected by Morris water maze 5 days after reperfusion. The number and structure of neurons and neuronal apoptosis in hippocampal were evaluated by Nissl staining. Results： Compared with Sham group,learning and memory capacity of SD rats in Model group declined significantly and the number of functional neurons in hippocampal decreased dramatically. Conelusion：TSG has ther- apeutic effects on epilepsy-rats,especially on delayed neuron apoptosis in hippocampal.