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Beneficial role of adipose-derived mesenchymal stem cells from microfragmented fat in a murine model of duchenne muscular dystrophy |
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| Authors: | Adrien Bouglé MD PhD Pierre Rocheteau PhD David Briand MS David Hardy PhD Franck Verdonk MD PhD Carlo Tremolada MD Mikael Hivelin MD PhD Fabrice Chrétien MD PhD |
| Institution: | 1. Infection and Epidemiology Department, Experimental Neuropathology Unit, Institut Pasteur, Paris, France
Sorbonne Université, Assistance Publique - Hôpitaux de Paris, Department of Anesthesiology and Critical Care Medicine, Pitié-Salpêtrière Hospital, Paris, France Assistance Publique-Hôpitaux de Paris, Paris, France Department of Anesthesiology and Critical Care Medicine, Institute of Cardiology, Pitié-Salpêtrière Hospital, Paris, France;2. Infection and Epidemiology Department, Experimental Neuropathology Unit, Institut Pasteur, Paris, France Service Hospitalo-Universitaire, Centre Hospitalier Sainte Anne, Paris, France Laboratoire Universitaire de Neuropathologie, Centre Hospitalier Sainte Anne, Paris, France;3. Infection and Epidemiology Department, Experimental Neuropathology Unit, Institut Pasteur, Paris, France;4. Infection and Epidemiology Department, Experimental Neuropathology Unit, Institut Pasteur, Paris, France Sorbonne Université, Assistance Publique - Hôpitaux de Paris, Department of Anesthesiology and Critical Care Medicine, Pitié-Salpêtrière Hospital, Paris, France Assistance Publique-Hôpitaux de Paris, Paris, France Department of Anesthesiology and Critical Care Department, Saint-Antoine Hospital, Paris, France;5. Image Institute, Milan, Italy;6. Assistance Publique-Hôpitaux de Paris, Paris, France Descartes University, Assistance Publique - Hôpitaux de Paris, Department of Plastic Surgery, Hôpital Européen Georges Pompidou, Paris, France Department of Plastic Surgery, Hôpital Européen Georges Pompidou, Paris, France |
| Abstract: | Introduction: No etiologic therapy is available for Duchenne muscular dystrophy (DMD), but mesenchymal stem cells were shown to be effective in preclinical models of DMD. The objective of this study is to investigate the effect of microfragmented fat extracted on a murine model of DMD. Methods: Fat tissue was extracted from healthy human participants and injected IM into DMD mice. Histological analysis, cytokines, and force measurement were performed up to 4 weeks after injection. Results: Duchenne muscular dystrophy mice injected with microfragmented fat exhibited an improved muscle phenotype (decreased necrosis and fibrosis), a decrease of inflammatory cytokines, and increased strength. Discussion: Administration of microfragmented fat in key muscles may improve muscular phenotype in patients with DMD. Muscle Nerve, 2019 |
| Keywords: | adipose-derived mesenchymal stem cells Duchenne muscular dystrophy mesenchymal stem cells microfragmented fat stem cells |