Consecutive Gas Chromatographic Determination of Phenytoin, Phenobarbital, Primidone, Phenylethylmalondiamide, Carbamazepine, Trimethadione, Dimethadione, Ethosuximide, and Valproate from the Same Serum Specimen |
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Authors: | Wolfgang Löscher Wolfgang Göbel |
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Institution: | Laboratorium für Pharmakologie unci Toxikologie, Fachbereich Veterincirmedizin, Freie Universität Berlin, Koserstrasse 20, D 1000, Berlin 33, West Germany |
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Abstract: | A quantitative gas-liquid chromatographic procedure is described for the consecutive determination of phenytoin, phenobarbital, primidone, phenylethylmalondiamide, carbamazepine, trimethadione, dimethadione, ethosuximide and valproate from a single serum specimen of 1.2 ml. After extraction from serum by two different procedures, the anticonvulsants are chromatographed without further purification on a 3% OV 17 column either with or without derivative formation by means of "on-column" methylation. Multiple internal standards are employed in order to enhance the reproducibility of drug-concentration measurement. |
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Keywords: | Gas chromatography Consecutive determination of anticonvulsant drugs |
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