顽固性颞叶癫痫患者海马或颞叶BDNF及其受体TrkB的测定

目的检测脑源性神经营养因子(BDNF)及其受体酪氨酸激酶B受体(TrkB)在难治性颞叶癫痫(TLE)患者颞叶和/或海马中的含量,探讨其在癫痫发病机制中的作用。方法选取经手术治疗的82例难治性TLE患者术中切除的海马或颞叶脑组织,用免疫组化方法对BDNF及其受体TrkB含量进行检测,并与11例对照进行比较。结果在难治性TLE患者中,BDNF在颞叶和海马中含量明显增加(分别P<0.05,P<0.01),且海马中含量明显高于颞叶(P<0.01);TrkB在颞叶和海马中含量显著增加(P<0.01),且海马中含量高于颞叶(P<0.05)。结论难治性TLE患者海马和颞叶中BDNF和TrkB含量增高,可能在癫痫发生、发展中起重要作用。

Content of BDNF and Its Receptor TrkB in the Temporal Lobe or Hippocampus of Intractable Temporal Lobe Epilepsy

Objective To study the content of brain derived neurotrophic factor(BDNF)and its receptor-tyrosine kinase receptor B(TrkB)in the temporal lobe and hippocampus of patients with intractable temporal lobe epilepsy(TLE)and discuss its function in the pathogenesis.Methods Immunohistochemical assay was performed to test the content of BDNF and TrkB in the surgically removed hippocampus and temporal lobe of patients with intractable temporal lobe epilepsy(n=82).The results were compared with controls(n=11).Results The content of BDNF was significantly increased in temporal lobe cortexes(P<0.05)and the hippocampus(P<0.01)in intractable temporal lobe epilepsy.The content of TrkB was significantly increased in the hippocampus(P<0.01)and the temporal lobe cortexes(P<0.01).The content of BDNF and TrkB was increased(P<0.01 and P<0.05 respectively)in the hippocampus compared with temporal lobe cortexes.Conclusions The results indicated that BDNF and TrkB,overincreased in the temporal and hippocampus of patients with intractable temporal lobe epilepsy,may play an important role in the generation and development of epilepsy.