α7烟碱乙酰胆碱受体基因与精神分裂症及吸烟行为的关系

目的 探讨α7烟碱样乙酰胆碱受体(nAchR)基因多态性与精神分裂症及吸烟行为的关系.方法 连续收集符合美国精神障碍诊断与统计手册第4版(DSM-IV)诊断标准的精神分裂症患者221例(患者组)和患者亲属432名(亲属组),评定患者吸烟情况;采用聚合酶链反应和限制性酶切技术,检测α7nAchR基因的rs1355920单...

Association study of a7 nicotinic acetylcholine receptor subunit gene with schizophrenia and smoking

Objective To investigate the associations of two polymorphisms in α7 nicotinic acetylcholine receptor subunit gene(α7 nAchR)with schizophrenia and smoking.Methods Eligible patients with schizophrenia(n=221)according to the Diagnostic and Statistical Manual of Mental Disorders Fouah Edition(DSM-IV)and their healthy relatives(n=432)were continuously recruited.Smoking data were collected in the patients.Two polymorphisms(rs1355920 and D15S1360)of α7 nAchR gene were genotyped using PCR-RFLP.Associations of the polymorphisms with schizophrenia and smoking were analyzed using transmission disequilibrium tests(TDT)and haplotype analysis.Results The odds of patient group received from parents allele A of SNP rs1355920 was increased(Z=2.56,P=0.010),and the odds of received allele G was decreased(Z=-2.70,P=0.007).The allele A of rs1355920 was more common in the patients than in the relatives(x2=6.910,P=0.008).The frequencies of D15S1360 were not significantly different between patients and relatives(x2=1.33 1,P=0.5 14).The haplotypes of the two locus were related to schizophrenia(x2=6.991,P=0.030).In addition,all these alleles or genotype frequencies of rs1355920 and D15S1360 did not differ significantly between smokers and non-smokers(P>0.05).Conclusion The α7nAchR gene may be associated with schizophrenia.but not with smoking.