| 人脑低级别星形细胞瘤及瘤周脑组织的差异蛋白表达 |
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| 引用本文： | 熊光仲,肖惠生,卢俊贤,张东山,毕长龙,彭利,熊舸,熊力,陈平,梁宋平.人脑低级别星形细胞瘤及瘤周脑组织的差异蛋白表达[J].中国神经再生研究,2010,5(24):1915-1920. |
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| 作者姓名： | 熊光仲 肖惠生 卢俊贤 张东山 毕长龙 彭利 熊舸 熊力 陈平 梁宋平 |
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| 作者单位： | 湖南师范大学生命科学学院,Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China,College of Life Science, Hunan Normal University, Changsha 410081, Hunan Province, China,Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China,Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China,College of Life Science, Hunan Normal University, Changsha 410081, Hunan Province, China,Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China,Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China,College of Life Science, Hunan Normal University, Changsha 410081, Hunan Province, China,College of Life Science, Hunan Normal University, Changsha 410081, Hunan Province, China |
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| 摘 要： |
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| 关 键 词： | 星形细胞瘤，神经胶质瘤，蛋白质组学，二维凝胶电泳 质谱 |
| 收稿时间： | 3/9/2010 12:00:00 AM |
| 修稿时间： | 3/9/2010 12:00:00 AM |
Differential protein expression in low-grade astrocytomas and peritumoral human brain tissues |
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| Xiong Guangzhong,Xiao Huisheng,Lu Junxian,Zhang Dongshan,Changlong Bi,Peng Li,Xiong Ge,Xiong Li,Chen Ping and Liang Songping.Differential protein expression in low-grade astrocytomas and peritumoral human brain tissues[J].Neural Regeneration Research,2010,5(24):1915-1920. |
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| Authors: | Xiong Guangzhong Xiao Huisheng Lu Junxian Zhang Dongshan Changlong Bi Peng Li Xiong Ge Xiong Li Chen Ping and Liang Songping |
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| Institution: | Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China,Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China,College of Life Science, Hunan Normal University, Changsha 410081, Hunan Province, China,Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China,Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China,College of Life Science, Hunan Normal University, Changsha 410081, Hunan Province, China,Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China,Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China,College of Life Science, Hunan Normal University, Changsha 410081, Hunan Province, China,College of Life Science, Hunan Normal University, Changsha 410081, Hunan Province, China |
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| Abstract: | Differential protein expression between various pathological grades of glioma has been shown in studies of glioma proteomics. However, very little data is available regarding normal brain tissues and glioma differential protein expression, because normal human brain tissues are difficult to harvest. The present study selected samples from low-grade astrocytomas and peritumoral brain tissues to analyze differential protein expression by two-dimensional (2D) electrophoresis and mass spectrometry techniques. Results revealing 36 protein spots by 2D electrophoresis, including 23 spots revealing increased expression and 13 spots revealing decreased expression. However, 25 differential proteins were identified by mass spectrometry, including 16 proteins with increased expression and 9 with decreased expression. Western blot analysis confirmed the mass spectrometry results, i.e., heat shock protein 70 (HSP70) and human transthyretin (TTR) expressions were increased, but glial fibrillary acidic protein (GFAP) was decreased, in astrocytomas. The present study constructed a 2D electrophoresis pattern between low-grade astrocytomas in the human brain and peritumoral tissues. Results demonstrated that a majority of differential proteins, such as HSP70, TTR, and GFAP, participate in malignant progression of gliomas. |
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| Keywords: | astrocytoma proteomics 2D gel electrophoresis mass spectrometry neural regeneration |
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