Neuroprotective effect of the Chinese medicine Tiantai No. 1 and its molecular mechanism in the senescence-accelerated mouse prone 8 |
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Authors: | Ying-hong Li Xu-sheng Wang Xiao-lin Chen Yu Jin Hong-bo Chen Xiu-qin Jia Yong-feng Zhang Zheng-zhi Wu |
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Institution: | 1. First Affliated Hospital of Shenzhen University Shenzhen Second People’s Hospital, Shenzhen, Guangdong Province, China;2. School of Life Sciences, Tsinghua University, Beijing, China;3. Guangzhou Medical University, Guangzhou, Guangdong Province, China;4. Shenzhen Key Laboratory of Health Sciences and Technology, Graduate School at Shenzhen, Tsinghua University, Beijing, China |
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Abstract: | Tiantai No. 1, a Chinese medicine predominantly composed of powderedRhizoma Gastrodiae,Radix Ginseng, and Ginkgo leaf at a ratio of 2:1:2 and dissolved in pure water, is neuroprotective in animal models of various cognitive disorders, but its molecular mechanism remains unclear. We administered Tiantai No. 1 intragastrically to senescence-accelerated mouse prone 8 (SAMP8) mice (a model of Alzheimer’s disease) at doses of 50, 100 or 150 mg/kg per day for 8 weeks and evaluated their behavior in the Morris water maze and expression of Alz-heimer’s disease-related proteins in the brain. Tiantai No. 1 shortened the escape latency in the water maze training trials, and increased swimming time in the target quadrant during the spatial probe test, indicating that Tiantai No. 1 improved learning and memory in SAMP8 mice. Immunohistochemistry revealed that Tiantai No. 1 restored the proliferation potential of Ki67-positive cells in the hippo-campus. In addition, mice that had received Tiantai No. 1 had fewer astrocytes, and less accumulation of amyloid-beta and phosphorylated tau. hTese results suggest that Tiantai No. 1 is neuroprotective in the SAMP8 mouse model of Alzheimer’s disease and acts by restoring neuronal number and proliferation potential in the hippocampus, decreasing astrocyte inifltration, and reducing the accumulation of amy-loid-beta and phosphorylated tau. |
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Keywords: | nerve regeneration neuroprotective effects Alzheimer’s disease Tiantai No 1 SAMP8 amyloid-beta autophagy-lysosome path-way ubiquitin proteasome pathway tau phosphorylation neuronal apoptosis astrocytosis neural regeneration |
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