The Third Hospital of Chinese PLA;The Third Hospital of Chinese PLA;The Third Hospital of Chinese PLA
Abstract:
BackgroundAbnormal changes in magnesium ion are closely related to cerebral injury. At present, some evidence indicates that magnesium reagent can improve nerve function and prognosis of patients with cerebral injury.ObjectiveTo observe the effect of magnesium sulfate on changes in nitric oxide synthase (NOS) activity in brain tissue of rats with acute craniocerebral injury.DesignCompletely randomized grouping design and randomly controlled study.SettingLaboratory of Neurosurgery, the Third Hospital of Chinese PLA.MaterialsFifty-four male SD rats of clean grade and weighing 220–250 g were randomly divided into normal control group (n =6), cerebral injury group (n =24) and magnesium sulfate group (n =24). Especially, rats in cerebral injury group and magnesium sulfate group were equally divided into four subgroups and observed at 0.5, 2, 6 and 24 hours after model establishment. A solution of 125 g/L of magnesium sulfate was provided by the Seventh Pharmaceutical Factory of Wuxi and the NOS assay kit by Nanjing Jiancheng Bioengineering Institute.MethodsThe experiment was carried out in the Institute of Neurosurgery, the Third Hospital of Chinese PLA from August 2000 to August 2002.