兔自体颌下腺部分移植模型的建立

背景：近年来临床上应用血管化自体颌下腺移植治疗重症角结膜干燥症取得了较好的治疗效果，但术后会出现泪溢的并发症，需要再次甚至多次手术切除部分腺体以减轻泪溢的症状。为减少自体颌下腺移植后出现的泪溢并发症，自体颌下腺部分移植是否可行尚待验证。 目的：通过建立兔颌下腺部分移植的动物实验模型，探讨兔颌下腺部分移植的可行性。 方法：取家兔左侧颌下腺2/3，应用显微外科血管吻合技术移植于颞侧皮下间隙，14 d后取出，苏木精-伊红染色观察其病理变化。 结果与结论：15只移植腺体存活兔中7只移植的颌下腺病理切片示正常，腺体正常度在30%以上的6只，腺体正常度在30%以下的2只。结果显示87%兔部分颌下腺移植后，保留了30%以上正常的腺体结构。兔部分颌下腺移植模型构建具可行性，该结果对采用自体颌下腺部分移植治疗干眼症的手术具有参考价值。 关键词：自体移植；颌下腺；兔；动物模型；移植动物模型

A rabbit model of partly autologous submandibular gland transplantation

BACKGROUND: Recently, vascularized autologous submandibular gland transplantation for treating severe keratoconjunctivitis sicca has achieved better therapeutic effects in clinic; however, dacryorrhea as postoperative complication was observed usually. Patients needed another or more operations to partly excise transplanted gland to reduce the symptom of dacryorrhea. For decreasing the complication of dacryorrhea post-operation, the feasibility of partly submandibular gland autotransplantation is still needed to be verified. OBJECTIVE: To investigate the feasibility of partly submandibular gland transplantation in rabbits. METHODS: The two third part of left submandibular gland was transfered to the space beneath temple skin with blood vessel anastomosis technique of microsurgery. At 14 days post-operation, the graft was taken out to observe pathological changes with HE staining. RESULTS AND CONCLUSION: Seven out of 15 survival rabbits had normal pathological structure of graft, and normal value of 6 cases was about 30% or even more, that of only 2 was less than 30%. The results suggested that partly submandibular gland transferring in rabbit model was feasibility and worth for treating dacryorrhea.