淫羊藿提取液对激素性股骨头坏死的作用

背景：研究表明中药淫羊藿可以改善激素性股骨头坏死的症状，但具体的作用机制尚未阐明。 目的：探讨淫羊藿提取液治疗激素性股骨头坏死的初步效果。 方法：清洁级SD成年雄性大鼠建立激素性股骨头坏死大鼠模型后，分别给予0.4，0.2 g/mL淫羊藿提取液或生理盐水干预，并设立未造模的空白对照组。干预8周后，检测各组大鼠血液三酰甘油、总胆固醇的变化情况，解剖出股骨头并切片光镜下观察骨密度变化情况，计算空骨陷窝比率，进行组间比较。 结果与结论：与空白对照组相比，模型组股骨头疏松，易于凿切，血脂升高，骨小梁变细、稀疏，空骨陷窝率增高，髓腔脂肪细胞增多；而淫羊藿高、低剂量组的上述病变情况有不同程度减低，以淫羊藿高剂量组病变程度最低。结果表明，淫羊藿提取液能够改善激素性股骨头坏死血流变因素，改善脂肪代谢紊乱，促进堆积脂肪代谢的作用；还能够促进体外成骨细胞增殖及分化成熟，抑制破骨细胞活性、促进股骨头骨再生，改变股骨头内环境向好的方向转变，防治激素引起的骨质疏松，为淫羊藿治疗激素性股骨头坏死提供了初步理论依据。

Effect of epimedium extract on the treatment of femoral head necrosis in rats

BACKGROUND: Studies have demonstrated that Chinese herb epimedium can improve steroid-induced necrosis of femoral head, but the specific mechanism remains unclear. OBJECTIVE: To explore the primary results of epimedium extract on the treatment of steroid-induced avascular necrosis of femoral head. METHODS: Male SD rats were prepared for rat models of steroid-induced avascular necrosis of femoral head and intervened by epimedium extract (0.4, 0.2 g/mL) or saline, at the same time, the rats without model preparation served as blank controls. The changes of blood glycerin, total cholesterol, and bone mineral density were observed at 8 weeks after operation. The spatial bone dimple ratio was calculated, and the differences were compared among groups. RESULTS AND CONCLUSION: Compared with the blank control group, the femoral head was loosened, the blood lipids were elevated, trabecular bone was thinning, sparse, the rate of empty lacunae was higher, and the medulla cavity fatty cells increased in the model group. But these lesions had varying degrees of reduction in the high and low-dose groups, and the pathological change of high-dose group was the lowest. Epimedium extract not only can improve blood flow changes of femoral head necrosis, improve metabolic disorder, promote the accumulation of fat metabolism, but also can promote the in vitro proliferation and differentiation into mature bone cells, inhibit osteoclast activity, promote femoral head bone regeneration and prevent steroid-induced necrosis of femoral head. The study provides a preliminary theoretical basis for the treatment of steroid-induced necrosis of femoral head using epimedium extract.