锂剂和氯胺酮对N2a细胞mTOR及GSK-3β的影响 |
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引用本文: | 石金云,李文媛,杨春,杨建军,周志强,徐建国.锂剂和氯胺酮对N2a细胞mTOR及GSK-3β的影响[J].神经疾病与精神卫生,2012,12(1):12-13. |
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作者姓名: | 石金云 李文媛 杨春 杨建军 周志强 徐建国 |
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作者单位: | 1. 210002,南京大学医学院临床学院南京军区南京总医院麻醉科 2. 南京大学医学院附属鼓楼医院麻醉科 |
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基金项目: | 国家自然科学基金,全军“十二五”科研面上项目资助 |
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摘 要: | 目的 观察锂剂和氯胺酮分别对N2a细胞中西罗莫司靶蛋白(mTOR)及糖原合酶激酶-3β( GSK-3β)的影响.方法 将N2a细胞随机分为3组(n=6),分别加入DMEM培养液(C组)、3μmol/L氯化锂(L组),1μmol/L氯胺酮(K组),孵育2d后采用Western Blotting分别检测mTOR和GSK- 3β表达.结果 与C组相比,L组和K组mTOR表达显著上调,GSK- 3β表达显著下调(P<0.05).结论 锂剂和氯胺酮可上调mTOR,下调GSK- 3β.
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关 键 词: | 锂 氯胺酮 西罗莫司靶蛋白 糖原合成酶激酶-3β N2a细胞 |
Effects of lithium and ketamine on the expression of mTOR and GSK-3β in N2a cells |
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Institution: | SHI Jin-yun ,LI Wen-yuan , YANG Chun , et al. Department of Anesthesiology, School of Medicine, Nanjing University, Nanjing General Hospital of Nanjing Military Command ,PLA, Nanjing 210002, China |
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Abstract: | Objective To evaluate the effects of lithium and ketamine on the expression of mammalian target of rapamycin (mTOR) and glycogen synthase kinase-3 beta (GSK-3β) in N2a cells. Methods N2a cells were randomly divided into 3 groups (n = 6) . DMEM culture solution (group C), lithium chloride 3/Lmol/L(group L) and ketamine 1 μmol/L(group K). Western Blotting was used to determine the expression of mTOR and GSK-3β in N2a cells 2 d after incubation. Results Compared with group C,group L and group K both had up-regulated mTOR and down-regulated GSK-3β (P 〈 0. 05). Conclusions lithium and ketamine could mediate the up-regulation of roTOR and down-regulation of GSK-3β. |
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Keywords: | Lithium Ketamine Sirolimus Glycogen synthase kinase-3β |
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