5-羟色胺1A受体激动剂8-OH-DPAT对癫痫合并抑郁神经发生的研究

目的探讨5-羟色胺1A(5-HT1A)受体与匹罗卡品诱导的癫痫大鼠合并抑郁海马齿状回神经发生的关系。方法从匹罗卡品诱导的慢性自发性颞叶癫痫大鼠中筛选出合并抑郁的大鼠3 2只,随机分成模型组、卡马西平(CBZ)组、CBZ+8-OH-DPAT低剂量(0.1 mg/kg)组、CBZ+8-OH-DPAT高剂量(1.0 mg/kg)组,每组8只。对照组8只,注射生理盐水(1 0 m l/kg)。药物干预后,制备大鼠脑片,利用免疫组织化学方法检测大鼠的神经发生。结果模型组海马齿状回神经发生较对照组明显增多,差异有统计学意义(P<0.0 5)。CBZ组、CBZ+8-OH-DPAT低剂量组、CBZ+8-OH-DPAT高剂量组较模型组神经发生明显增多,差异有统计学意义(P<0.0 5)。CBZ+8-OH-DPAT高剂量组较CBZ组、CBZ+8-OH-DPAT低剂量组神经发生明显增多,差异有统计学意义(P<0.0 5)。但CBZ组与CBZ+8-OH-DPAT低剂量组比较神经发生的差异没有统计学意义(P>0.0 5)。结论高剂量的5-HT1A受体激动剂8-OH-DPAT在实验的过程中能够增加癫痫合并抑郁大鼠的神经发生。

Effect of 5-hydroxytryptamine 1A receptor activator 8-OH-DPAT on neurogenesis in epileptic rats with depression

Objective To explore the correlation between 5-hydroxytryptamine(5-HT)1A receptors and hippocampal dentate gyrus neurogenesis in pilocarpine-induced epileptic rats with depression.Methods Thirty-two rats with depression which were selected from pilocarpine-induced spontaneous temporal lobe epileptic rats were randomly divided into 4 groups(n=8 each): model,CBZ,CBZ+8-OH-DPAT low dose(0.1 mg/kg) and CBZ+8-OH-DPAT high dose(1.0 mg/kg).The rats receving an injection of normal saline were used as the control group.Neurogenesis in the brain tissue was determined with immunohistochemistry.Results The hippocampal dentate gyrus neurogenesis increased significantly in the model group compared with that in the control group(P<0.05).The CBZ and the CBZ+8-OH-DPAT low dose and high dose groups showed increased neurogenesis compared with the model group(P<0.05).More remarkably increased neurogenesis was found in the CBZ+8-OH-DPAT high dose group compared with the CBZ and the CBZ+8-OH-DPAT low dose groups(P<0.05).There were no significant differences in the neurogenesis between the CBZ+8-OH-DPAT low dose and the CBZ groups.Conclusions High doses of 8-OH-DPAT can increase neurogenesis in epileptic rats with depression.