芹菜素对脑胶质瘤细胞U87增殖、侵袭及迁移能力的影响

目的 探讨芹菜素对脑胶质瘤U87细胞增殖、侵袭及迁移能力的影响及作用机制。方法 体外培养脑胶质瘤U87细胞，加入20、40、80 μmol/L芹菜素干预，以培养基作为空白对照组。使用MTT法检测U87细胞的增长抑制率，使用Hocst染色法观察细胞凋亡情况；使用Transwell检测U87细胞侵袭能力；划痕实验法检测U87细胞迁移能力；使用免疫印迹法检测U87细胞基质金属蛋白酶（MMP）-2、MMP-9、Bak1、Caspase-3表达水平。结果 与空白对照组比较，芹菜素组细胞抑制率显著增加（P<0.05）、细胞凋亡数目明显增加（P<0.05）、细胞侵袭数目显著下降（P<0.05）、细胞迁移数目明显减少（P<0.05），U87细胞MMP-2、MMP-9、Bak1蛋白表达显著下降（P<0.05），Caspase-3蛋白表达显著升高（P<0.05）；而且均呈浓度依赖性。结论 芹菜素可有效抑制脑胶质瘤U87细胞增殖、促进凋亡，可能与下调MMP-2、MMP-9、Bak1蛋白表达水平，上调Caspase-3表达有关。

Effects of apigenin on proliferation,invasion and migration of glioma cell line U87 cells

Objective To explore the effects of apigenin on the proliferation, invasion and migration of glioma cell line U87 cells and their mechanism. Methods U87 cells were cultured in vitro and then were divided into blank control group, experimental group I in which U87 cells were treated with low-dose apiginen (20 μmol/L), experimental group Ⅱ in which the cells were treated with medium-dose apigenin (40 μmol/L) and experimental group Ⅲ in which the cells were treated with high-dose apigenin (80 μmol/L). The U87 cells proliferation, apoptosis, and invasive and migration abilities were determined respectively by MTT, Hocst staining, transwell test and scratch assay. The levels of matrix metalloproteinase (MMP)-2, MMP-9, Recombinant Bcl2 Antagonist/Killer 1 (Bak1), cysteinyl aspartate specific proteinase-3 (Caspase-3) expressions in the U87 cells were detected by Western blotting. Results Compared with the blank control group, the abilities of U87 cells proliferation, migration and invasion were significantly 1nhibited in all the experimental groups (P<0.05). The rates of the inhibition of U87 cells proliferation, migration and invasion were positively related to the dose of apigenin used to treat the U87 cells (P<0.05). Compared with the blank control group, in the U87 cells the expressions of MMP-2, MMP-9 and Bak1 in the U87 cells were significantly inhibited and the protein expression of Caspase-3 protein was significantly up-regulated in all the experimental groups (P<0.01), The levels of MMP-2, MMP-9 and Bak1 protein expression in the U87 cells were negatively related to the dose of apigenin used to treat the U87 cells and the level of Caspase-3 protein expression in the U87 cells was positively related to the dose used to treat the U87 cells (P<0.01). Conclusion It is suggested that apigenin can inhibit the proliferation, invasion and migration of glioma cell line U87 cells possibly by the down-regulation of the levels of MMP-2, MMP-9 and Bak1 protein expression and up-regulate the level of Caspase-3 protein expression.