NSCs在OECs诱导下定向分化及神经元电生理特性研究

目的探索大鼠嗅鞘细胞（OECs）对神经干细胞（NSCs）分化的影响，并记录分化后神经元电生理特性。方法在无血清改良Eagle培养基（DMEM／F12）培养的NSCs中，加入OECs条件培养基，观察分化情况，巢蛋白（nestin）鉴定NSCs、神经丝蛋白200（NF200）鉴定神经元、膜片钳检测神经元电生理特性。结果OECs能促进NSCs分化为神经元，分化后的神经元记录到快速激活快速失活、能被河豚毒素（TTX）特异阻断的钠电流及慢激活慢失活、能被四乙基氯化铵（TEA）特异阻断的延迟整流性钾电流。结论OECs能诱导NSCs分化，分化后的神经元具有活跃的电生理特性，具有替代凋亡、坏死神经元的潜能。

Differentiation of neural stem cells and their electrophysiological properties after neural differentiation treated with olfactory ensheathing cells

Objective To study the differentiation of neural stem cells （NSCs） and their electrophysiological properties after neural differentiation treated with olfactory ensheathing cells （OECs）. Methods NSCs cultured in DMEM/F12 serum-free medium were induced to differentiate to neurons by adding OEC-conditioned medium. Nestin was used to identify NSCs and neurofilament 200 （NF200） was used to identify neurons. The patch clamp was used to detect the electrophysiological properties of differentiated neurons. Results OECs could promote the differentiation of NSCs into neurons. Some neurons displayed fast etrodotoxin （Trx）- sensitive Na ^＋ current, as well as slow tetraethylammonium chloride （TEA） -sensitive delayed-rectifier K ^＋ current. Conclusion OECs can promote the differentiation of NSCs. The NSCs-differentiated neurons have active electrophysiological properties, which are potential to replace the apoptotic or necrotic neurons.