rTMS促进大鼠局灶性脑缺血海马内源性神经干细胞分化的研究

目的探讨重复经颅磁刺激（rTMS）对局灶性脑缺血大鼠海马内源性神经干细胞分化的影响。方法线栓法制备大鼠大脑中动脉闭塞（MCAO）模型，随机分为脑缺血自然恢复组和rTMS治疗组，用荧光显微镜和共聚焦显微镜观察缺血14d、28d后各组大鼠海马中5-溴脱氧尿核苷（BrdU）与神经元特异核蛋白（NeuN）、神经胶质酸性蛋白（GFAP）共同标记的阳性细胞，并在高倍荧光显微镜下对双标阳性细胞计数。结果脑缺血后14d、28d，rTMS治疗组大鼠海马BrdU／NeuN双标阳性细胞数量分别为17．12±2．91、23．20±5．97，较相应自然恢复组12．96±2．79、15．92±2．52明显增加，两组同一时间点组间比较有统计学差异（P〈0．01）。而脑缺血后14d、28d，rTMS治疗组大鼠海马BrdU／GFAP双标阳性细胞数量分别为30．48±4．58、36．48±4．90，较相应自然恢复组37．44±3．58、43．60±5．96减少，两者相比有统计学差异（P〈0．05）。结论局灶性脑缺血大鼠海马增殖的内源性神经干细胞，可分化为神经元或神经胶质细胞，而rTMS可促进海马内源性神经干细胞向神经元的分化。

Effect of rTMS on the differentiation of endogenous neural stem cells of the hippocampus in rats with focal cerebral ischemia

Objective To investigate the effect of repetitive transcranial magnetic stimulation(rTMS) on the differentiation of endogenous neural stem cells in the hippocampus of rats with focal cerebral ischemia. Methods Focal ischemia-reperfusion models were established in SD rats by middle cerebral artery occlusion (MCAO). Rats were randomized to the rTMS+ischemia group and the ischemia-only group. The 5-bromodeoxyuridine (BrdU)/neuron-specific nuclear protein (NeuN) and BrdU/ glial fibrillary acidic protein (GFAP) ...