原发性脑创伤后人脑皮层差异蛋白质组研究

目的应用蛋白质组学技术，研究不同程度脑创伤后的人脑皮层蛋白质组表达变化的情况。方法将临床标本以GCS评分分为中度和重度损伤组，提取脑挫伤部位皮层的总蛋白。通过双向电泳．分离蛋白。应用胶内酶切、生物质谱，鉴定由图像分析软件所得出的具有表达差异的蛋白质点。结果通过比较，目前已发现12个蛋白质点，表达水平具有显著性差异变化。在已鉴定出的蛋白点中，属于10种蛋白质。依其功能可分为：细胞骨架、代谢反应、氧化应激反应、功能未知等几类。在重伤组中，一共有9个蛋白点、6种蛋白表达上调（α-烯醇化酶、磷酸丙糖异构酶、5’磷酸吡哆醇氧化酶、crtstalin、stathmin-1、NP25）；中度损伤组3个蛋白点、4种蛋白表达上调（谷氨酰胺S转移酶、5’3’核苷酸酶、HSPC108、proapolipoprotein）。结论外伤后，神经系统由于受伤程度的不同，蛋白表达水平会发生变化。原发性脑创伤的损伤程度不同将产生神经系统病生理反应的差异。

The research of the differential human cortex proteome after primary mechanical traumatic brain injury

Objective According to the different severity of primary traumatic brain injury, to elevate the differential protein expression levels of human brain cortex with proteomics technique between moderate and severe traumatic brain injury. Methods 15 appropriate injured samples selected in this study were divided into moderate and severe injury group, all of them were extracted the total proteins. The total proteins mixture extracted in protein lysate were separated with two dimensional gel electrophrosis. The differential protein spots analyzed by ImageMaster 5.0 software were identified by matrix -assisted laser desorption/ionization -time of flight mass spectrometry (MALDI-TOF MS) . Results After analyzing 2-D images with software, we found 12 protein spots showed significant expression levels change. The differential protein spots identified by mass spectrometry belonged to 10 products of protein, which were divided into several functional group: eyctoskeleton, intermediary metabolism, and unknown function etc. Conclusions The proteomics technique including 2D -electrophoresis and MS provide us a high-throughout tool to research the global protein in brain. The human cortical proteome map provides a analytical reference for the protein expression in the brain area in health and disease. In acute phase of post-trauma, the significant changes happen in the impaired brain area, especially intermediary metabolism enzyme proteins. The differential severity of primary mechanical TBI can cause the different response of CNS pathophysiology.