转基因组织工程骨体内移植修复颅骨缺损的实验研究

目的 采用血管内皮生长因子(VEGF)转基因组织工程骨对兔颅骨缺损模型进行修复,对转基因技术在颅骨组织工程方面的应用进行初步探讨.方法 建立兔颅骨缺损模型,以转基因MSCs与成骨诱导后的MSCs混合作为种子细胞构建的组织工程骨进行移植修复,以单纯组织工程骨为对照.通过血生化检查、组织学观察、X线检查以及组织学评分等指标来判断修复效果.结果 转基因组织工程骨较单纯组织工程骨成骨细胞生长、增殖、血管形成更活跃,其成骨能力明显增强.结论 VEGF转基因组织工程骨能加快修复区的骨形成,可望为临床大块颅骨缺损修复提供有效方法.

Study on the repair effects of skull defect with VEGF transgenetic tissue engineering skull

Objective VEGF transgenetic tissue engineering bone was used to repair rabbit skull defect model ,which aimed to explore the effects of trasgene technique in skull tissue engineering. Method Rabbit skull defect model was made by resecting 15 mm×15 mm middle skull segments. Seed cells were formed by mixing transgenetic MSCs with osteogenic induced MSCs, and then these seed cells were used to build tissue engineering skull and then the constructed engineering skull was used to transplant the defected rabbit skull. Tissue engineering skull made by osteogenic MSCs was regarded as the control group. Repair effects of defected skull were assessed by blood biochemical test, histology investigation, X-ray examination and histology score at different time after transplantation. Results Compared with the ostengenic MSCs skull ,transgenetic tissue engineering skull showed more active vascularization and osteogenesis. The repair effects in trasgenetic group were more obvious than the control group at the same period. Condusions VEGF transgenetic tissue engineering skull showed rapid vascularization, osteogenesis and bone formation, which would provide a promising strategy for large segment skull defect repairing in clinics.