| 无抽搐电休克对抑郁大鼠学习记忆功能的影响及其突触可塑性机制 |
| |
| 引用本文： | 刘媛媛,闵苏,董军,曹俊,黎平,刘永峰.无抽搐电休克对抑郁大鼠学习记忆功能的影响及其突触可塑性机制[J].中国神经精神疾病杂志,2010,36(2). |
| |
| 作者姓名： | 刘媛媛 闵苏 董军 曹俊 黎平 刘永峰 |
| |
| 作者单位： | 重庆医科大学附属第一医院麻醉科,重庆,400016 |
| |
| 基金项目： | 国家自然科学基金项目 |
| |
| 摘 要： | 目的探讨无抽搐电休克对抑郁大鼠学习记忆功能的影响及其突触可塑性机制。方法SD大鼠50只随机分为无抽搐电休克(MECT)组、电休克(ECT)组、丙泊酚组、抑郁模型组(简称抑郁组)、正常对照组(简称对照组)各10只,前4组采用孤养结合慢性不可预见轻度应激制备抑郁模型。建模成功后,MECT组和ECT组每日1次分别腹腔注射丙泊酚和生理盐水后给予电刺激,丙泊酚组腹腔注射丙泊酚但不给予电刺激,连续7d。采用openfield和Morris水迷宫分别评定行为学和学习记忆功能的改变,免疫组织化学法和逆转录聚合酶链反应检测海马突触素(SYP)及其mRNA的表达。结果治疗后MECT组和ECT组的openfield评分均分别明显高于丙泊酚组和抑郁组,差异有统计学意义(P0.05);ECT组Morris水迷宫测验的逃避潜伏期较MECT组、丙泊酚组及抑郁组延长而空间探索时间缩短(P0.05);MECT组和ECT组较丙泊酚组、抑郁组SYP蛋白及其mRNA表达明显升高(P0.05),而MECT组较ECT组的表达减少(P0.05)。结论丙泊酚可改善抑郁大鼠电休克后学习记忆功能,其机制可能与抑制电休克后海马SYP的过度表达有关。
|
| 关 键 词： | 抑郁症 无抽搐电休克 突触素 突触可塑性 |
Effect of MECT on learning memory in depressed rats and its synaptic plasticity mechanism |
| |
| LIU Yuanyuan,MIN Su,DONG Jun,CAO Jun,LI Ping,LIU Yongfeng.Effect of MECT on learning memory in depressed rats and its synaptic plasticity mechanism[J].Chinese Journal of Nervous and Mental Diseases,2010,36(2). |
| |
| Authors: | LIU Yuanyuan MIN Su DONG Jun CAO Jun LI Ping LIU Yongfeng |
| |
| Institution: | LIU Yuanyuan,MIN Su,DONG Jun,CAO Jun,LI Ping,LIU Yongfeng.Department of Anesthesiology,the First Affiliated Hospital of Chongqing Medical University.No.1 Youyi Road,Yuzhong District,Chongqing 400016,China |
| |
| Abstract: | Objective To explore the effect of MECT on learning memory in depressed rats and its synaptic plasticity mechanism.Methods Fifty SD rats were randomly divided into 5 groups(n=10):MECT (received ECT with intraperitoneal propofol),ECT (received ECT only),propofol (received intraperitoneal propofol),depression,and control.The treatments were given daily for 7 consecutive days.All rats underwent open field and Morris water maze test.The SYP protein and mRNA expressions in rat hippocampus were detected using imm... |
| |
| Keywords: | Depression Electroconvulsive therapy Synaptophysin Synaptic plasticity |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
