血管内尼龙线栓塞大鼠大脑中动脉局灶性脑缺血模型的改进

目的 确立更规范统一的制作大鼠局灶性脑缺血模型方法，使脑梗死体积更加稳定。方法 对24只大鼠使用液态硅胶涂层尼龙线栓塞大脑中动脉，分别缺血l，2，6和24h后再灌注24h，监测缺血侧局部脑血流，测定脑梗死体积及脑水肿程度。结果 缺血后所有大鼠局部脑血流均降到缺血前基值的25％以下，TTC染色显示所有动物在缺血侧皮质和尾状核均有明显的梗死灶和缺血，缺血1h组梗死体积与缺血2h以上组有显性差异，缺血2h以上各组之间梗死体积无显性差异；各组脑水肿程度无显性差异。结论 应用硅胶涂层尼龙线结合局部脑血流监测，缺血2h以上同时予以血流监测，可制作梗死体积稳定的大鼠局灶性脑缺血模型。

Modified focal ischemic model of rat middle cerebral artery occlusion using nylon suture embolization

Objective To determine modified method for rat focal ischemia model with more stable infarct volume. Methods The middle cerebral arteries were embolized with nylon suture coated with liquid silicon glue in 24 rats. After 1, 2, 6 and 24 hours ischemia and 24 hours reperfusion, the regional cerebral blood flow was monitored and infarct volume and extent of brain edema were determined. Results After ischemia, the regional cerebral blood flow reduced below 25% of baseline in all rats. TTC stain revealed infarction or ischemia in cortex and caudaputaman of occluded sides in all rats. There is a significant difference between 1-hour ischemia group and other groups. No significant difference was found between 2, 6 and 24 hours groups. There is no significant difference in edema extent among each group. Conclusion Combined with regional cerebral blood flow monitoring, liquid silicon glue coated nylon suture embolization for more than 2 hours can produce stable infarct volume rat focal ischemia model.