COX-2和iNOS在喉鳞癌中的表达及其与血管生成的相关研究

目的探讨喉鳞癌组织中环氧合酶-2（COX-2）和诱导型一氧化氮合酶（iNOS）的表达及其相关性，同时探讨二者与喉鳞癌血管生成的关系。方法应用免疫组化检测48例喉鳞癌组织、20例癌旁组织及20例声带息肉组织中COX-2和iNOS的表达，并观察微血管密度（MVD）。结果COX-2和iNOS在喉鳞癌组织中表达上调，与癌旁组织及声带息肉组织相比均有统计学意义（P〈0．05）。COX-2蛋白表达与喉鳞癌患者肿瘤临床分期有关。iNOS蛋白表达与喉鳞癌患者淋巴结转移、肿瘤病理分期有关。COX-2和iNOS蛋白表达之间呈正相关，两种蛋白在喉鳞癌中的表达有协同关系。喉鳞癌组织中MVD表达与声带息肉组织、癌旁组织有显著差异，且COX-2、iNOS与MVD显著正相关。结论COX-2和iNOS可能在喉鳞癌发生发展中起一定的促进作用。喉鳞癌组织MVD增高与COX-2及iNOS有关，COX-2与iNOS可能通过促进新生血管形成共同影响喉鳞癌的发生发展。

Expression of cyclooxygenase-2 and inducible nitric oxide synthase in laryngeal squamous cell carcinoma and their correlation with angiogenesis of the tumor

Objective To study the expression of cyclooxygenase- 2 （ COX- 2 ） and inducible nitric oxide synthase （iNOS） in laryngeal squamous cell carcinoma （LSCC）, and to discuss the correlation between the expression and the angiogenesis of LSCC. Methods 48 samples of LSCC, 20 samples of squamous epidermis adjacent to the tumor, and 20 samples of vocal polyp were collected. The expression of COX-2 and iNOS was detected with S- P immunobistochemical staining, and the microvessel density （ MVD ） of all the samples was measured as well. Results The expression of COX-2 and iNOS in LSCC was up-regulated significantly compared with either that of squamous epidermis adjacent to the tumor or that of vocal polyp （ P 〈 0.05 ） . The expression of COX- 2 was correlated with the TNM stage of tile tumor. The expression of iNOS was correlated with the pathologigal stage of the tumor and the lymphatic metastasis. The expression of COX-2 was positively correlated with that of iNOS （ r =0.490, P 〈0.01）. The difference between the MVD of LSCC and that of squamous epidermis adjacent to tile tumor or that of vocal polyp was statistically significant （ P 〈 0. 05 ） . The MVD was positively correlated with the expression of COX-2 and iNOS. Conclusion COX-2 and iNOS may facilitate the pathogenesis and development of LSCC via accelerating the angiogenesis of the tumor. The high MVD in LSCC may be related with the expression of COX-2 and iNOS.