| EGCG对鼻咽癌细胞株CNE-2及相关基因表达的影响 |
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| 引用本文： | 雷迅,孔中雨,范才文,易世江,向秋,刘强和.EGCG对鼻咽癌细胞株CNE-2及相关基因表达的影响[J].临床耳鼻咽喉头颈外科杂志,2012(15):697-700. |
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| 作者姓名： | 雷迅 孔中雨 范才文 易世江 向秋 刘强和 |
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| 作者单位： | 桂林医学院附属医院耳鼻咽喉-头颈外科;桂林医学院科学实验中心 |
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| 基金项目： | 广西科学技术厅科研基金资助课题(No:桂科自0640187);广西卫生厅科研基金资助课题(No:重200652) |
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| 摘 要： | 目的:研究EGCG对鼻咽癌细胞系CNE-2的增殖与凋亡作用,探讨其对细胞增殖与凋亡相关基因Bcl-2、Bax、Caspase-3表达的影响。方法:应用MTT实验、流式细胞仪检测细胞的增殖与周期;Hoechst33258荧光染色法检测细胞凋亡;RT-PCR检测Bcl-2、Bax、Caspase-3表达。结果:EGCG明显抑制CNE-2细胞的增殖,诱导其凋亡,该作用具有剂量-效应关系。EGCG抑制CNE-2细胞Bcl-2表达,诱导CNE-2细胞Bax、Caspase-3表达。结论:EGCG在体外具有抗鼻咽癌细胞的作用,此作用可能是通过调节细胞增殖与凋亡基因的表达实现的。
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| 关 键 词： | 表没食子儿茶素没食子酸酯 鼻咽肿瘤 CNE-2 |
Effects of EGCG on the nasopharyngeal carcinoma cell line CNE-2 and the expression of related gene |
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| LEI Xun,KONG Zhongyu,FAN Caiwen,YI Shijiang,XIANG Qiu,LIU Qianghe.Effects of EGCG on the nasopharyngeal carcinoma cell line CNE-2 and the expression of related gene[J].Journal of Clinical Otorhinolaryngology,2012(15):697-700. |
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| Authors: | LEI Xun KONG Zhongyu FAN Caiwen YI Shijiang XIANG Qiu LIU Qianghe |
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| Institution: | 1(1Department of Otorhinolaryngology Head and Neck Surgery,Affiliated Hospital of Guilin Medical College,Guilin,541001,China;2Center Laboratory of Guilin Medical College) Corresponding author:LEI Xun,E-mail:kongzhongyulql@163.com |
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| Abstract: | Objective:To study the effects of epigallocatechin-3-gallate(EGCG) on proliferation and apoptosis of nasopharyngeal carcinoma CNE-2 cell line and analyze the expression of Bcl-2,Bax and Caspase-3 in the cell line which treated with EGCG.Method:MTT assay and flow cytometry were used to analyze cell proliferation and cell cycle.Hoechst33258 fluorescence staining was adopted to study cell apoptosis.RT-PCR was used to detect the expression of Bcl-2,Bax,Caspase-3.Result:EGCG could significantly inhibit proliferation of CNE-2 cell line and induce its apoptosi with dose-independent relationship.EGCG could suppress the expression of Bcl-2 and induce expression of Bax,Caspase-3.Conclusion:EGCG in vitro has efficacy of anti-nasopharyngeal carcinoma cells,which may be through regulating the expression of cell proliferation and apoptosis genes involved. |
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| Keywords: | epigallocatechin-3-gallate nasopharyngeal neoplasms CNE-2 |
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